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Gibco™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein (PeproTech®) is a high-quality recombinant human basic Fibroblast Growth Factor produced in E. coli and purified to ≥95% by SDS-PAGE and HPLC. This biologically active 17.2 kDa protein is qualified for stem cell applications and is widely used in cell proliferation, differentiation, angiogenesis, and regenerative medicine research.
FGF-2 is a potent mitogenic and angiogenic growth factor that plays a critical role in embryonic development, tissue repair, stem cell maintenance, and tumor biology.
Key Features
- Recombinant human FGF-2 (154 amino acids)
- Molecular weight: 17.2 kDa
- High purity: ≥95%
- Endotoxin level: <1 EU/µg
- High bioactivity: ED50 ≤ 0.1 ng/mL
- Specific activity: ≥ 1 × 10⁷ units/mg
- Lyophilized, preservative-free
- Qualified for stem cell use
- Research Use Only (RUO)
Biological Function & Mechanism
FGF-2 binds to high-affinity Fibroblast Growth Factor Receptors (FGFRs) on the cell surface, triggering receptor dimerization and activation of intracellular signaling cascades.
Major downstream pathways include:
- MAPK/ERK signaling pathway
- PI3K–AKT pathway
- Cell survival and proliferation signaling
- Angiogenesis and tissue regeneration pathways
FGF2 exists in multiple isoforms with distinct intracellular localization. The 18 kDa cytosolic isoform primarily mediates paracrine and autocrine effects, while higher molecular weight isoforms can localize to the nucleus and exert intracrine functions.
Applications
- Stem cell culture and maintenance (PSC/iPSC)
- Embryonic stem cell expansion
- Mesenchymal stem cell (MSC) proliferation
- Cell differentiation studies
- Angiogenesis assays
- Wound healing research
- Tumor biology and cancer signaling studies
- Cell stimulation and induction assays
- Bioactivity and binding assays
Product Specifications
| Parameter | Specification |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Molecular Weight | 17.2 kDa |
| Amino Acid Length | 154 aa |
| Purity | ≥ 95% |
| Endotoxin | < 1 EU/µg |
| Form | Lyophilized |
| Storage | -20°C |
| Shipping | Ambient |
| Conjugate | Unconjugated |
Available Sizes
| Catalog Number | Quantity |
|---|---|
| 100-18B-10UG | 10 µg |
| 100-18B-50UG | 50 µg |
| 100-18B-100UG | 100 µg |
| 100-18B-250UG | 250 µg |
| 100-18B-500UG | 500 µg |
| 100-18B-1MG | 1 mg |
Gibco™ Human FGF-basic (FGF-2/bFGF) Recombinant Protein delivers high purity, low endotoxin levels, and exceptional bioactivity for advanced cell culture and regenerative research applications. Its well-characterized signaling properties and stem cell qualification make it a reliable choice for laboratories investigating proliferation, differentiation, angiogenesis, and tumor progression.
converting ed50 to ng-ml specific activity.pdf
recombinant proteins reconstitution and storage protocol flyer.pdf
Gibco™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein (PeproTech®) is a high-quality recombinant human basic Fibroblast Growth Factor produced in E. coli and purified to ≥95% by SDS-PAGE and HPLC. This biologically active 17.2 kDa protein is qualified for stem cell applications and is widely used in cell proliferation, differentiation, angiogenesis, and regenerative medicine research.
FGF-2 is a potent mitogenic and angiogenic growth factor that plays a critical role in embryonic development, tissue repair, stem cell maintenance, and tumor biology.
Key Features
- Recombinant human FGF-2 (154 amino acids)
- Molecular weight: 17.2 kDa
- High purity: ≥95%
- Endotoxin level: <1 EU/µg
- High bioactivity: ED50 ≤ 0.1 ng/mL
- Specific activity: ≥ 1 × 10⁷ units/mg
- Lyophilized, preservative-free
- Qualified for stem cell use
- Research Use Only (RUO)
Biological Function & Mechanism
FGF-2 binds to high-affinity Fibroblast Growth Factor Receptors (FGFRs) on the cell surface, triggering receptor dimerization and activation of intracellular signaling cascades.
Major downstream pathways include:
- MAPK/ERK signaling pathway
- PI3K–AKT pathway
- Cell survival and proliferation signaling
- Angiogenesis and tissue regeneration pathways
FGF2 exists in multiple isoforms with distinct intracellular localization. The 18 kDa cytosolic isoform primarily mediates paracrine and autocrine effects, while higher molecular weight isoforms can localize to the nucleus and exert intracrine functions.
Applications
- Stem cell culture and maintenance (PSC/iPSC)
- Embryonic stem cell expansion
- Mesenchymal stem cell (MSC) proliferation
- Cell differentiation studies
- Angiogenesis assays
- Wound healing research
- Tumor biology and cancer signaling studies
- Cell stimulation and induction assays
- Bioactivity and binding assays
Product Specifications
| Parameter | Specification |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Molecular Weight | 17.2 kDa |
| Amino Acid Length | 154 aa |
| Purity | ≥ 95% |
| Endotoxin | < 1 EU/µg |
| Form | Lyophilized |
| Storage | -20°C |
| Shipping | Ambient |
| Conjugate | Unconjugated |
Available Sizes
| Catalog Number | Quantity |
|---|---|
| 100-18B-10UG | 10 µg |
| 100-18B-50UG | 50 µg |
| 100-18B-100UG | 100 µg |
| 100-18B-250UG | 250 µg |
| 100-18B-500UG | 500 µg |
| 100-18B-1MG | 1 mg |
Gibco™ Human FGF-basic (FGF-2/bFGF) Recombinant Protein delivers high purity, low endotoxin levels, and exceptional bioactivity for advanced cell culture and regenerative research applications. Its well-characterized signaling properties and stem cell qualification make it a reliable choice for laboratories investigating proliferation, differentiation, angiogenesis, and tumor progression.
converting ed50 to ng-ml specific activity.pdf
recombinant proteins reconstitution and storage protocol flyer.pdf
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Spécifications
| Pack Size | 1mg, 50 µg, 100 µg, 10 /µg, 250 µg |