Gibco™ Human M-CSF Recombinant Protein

Gibco™ Human M-CSF (Macrophage Colony-Stimulating Factor) Recombinant Protein is a high-purity cytokine essential for the survival, proliferation, and differentiation of monocytes, macrophages, and osteoclast progenitor cells. Expressed in HEK293 cells, this recombinant protein closely mimics the natural glycosylation pattern, enhancing bioactivity and stability in cell culture applications.

M-CSF also plays a crucial role in vascular endothelial growth factor (VEGF) secretion, aiding in neovascularization and immune regulation. With an endotoxin concentration of <0.1 ng/µg, Gibco™ Human M-CSF is optimized for use in immunology, oncology, stem cell research, and drug discovery.

Key Features & Benefits

1. High Purity & Bioactivity
   • Purity >95% by SDS-PAGE, ensuring minimal contaminants.
   • Bioactive with ED50 ≤ 5 ng/mL, promoting dose-dependent proliferation of M-NSF60 cells.
2. Optimized for Functional Assays & Cell Culture
   • Supports monocyte-to-macrophage differentiation and osteoclast formation.
   • Induces VEGF secretion for endothelial cell mobilization.
3. HEK293-Expressed for Natural Glycosylation
   • Ensures higher stability and closer structural resemblance to native M-CSF.
   • Suitable for in vitro functional studies and immune assays.
4. Low Endotoxin for Consistency in Sensitive Applications
   • Endotoxin concentration of <0.1 ng/µg, minimizing immune response variability.
   • Ideal for macrophage activation and cytokine research.
5. Lyophilized for Long-Term Stability
   • Easily reconstituted in 20mM Tris-HCl, pH 8.0.
   • Can be stored at ≤ -20°C to -80°C in working aliquots to avoid freeze-thaw cycles.

Product Specifications

Use Cases & Applications

1. Immunology & Macrophage Differentiation

• Monocyte-to-Macrophage Differentiation
  • Stimulates monocyte proliferation and differentiation into macrophages.
  • Enhances immune response and antigen presentation in immunological studies.
• Macrophage Activation & Functional Assays
  • Induces VEGF secretion, supporting neovascularization.
  • Used in macrophage-mediated tumor immunity and inflammation research.

2. Stem Cell Research & Osteoclast Formation

• Hematopoietic Stem Cell (HSC) Differentiation
  • Supports differentiation of HSCs into monocyte-macrophage lineages.
  • Used in stem cell expansion protocols.
• Osteoclast Progenitor Differentiation
  • Promotes osteoclast development and bone resorption studies.
  • Used in bone metabolism and osteoporosis research.

3. Cancer Research & Tumor Microenvironment Studies

• Role in Tumor-Associated Macrophages (TAMs)
  • Supports studies on tumor progression and macrophage recruitment.
  • Facilitates tumor immunosuppression modeling.
• VEGF-Mediated Angiogenesis Research
  • Enhances endothelial cell mobilization and neovascularization.
  • Used in anti-angiogenic drug screening.

4. Drug Discovery & High-Throughput Screening

• Cytokine-Based Drug Screening Assays
  • Used in high-throughput screening of anti-inflammatory and immune-modulating drugs.
  • Helps evaluate therapeutic compounds affecting macrophage differentiation.
• Biomarker Discovery & Immune Response Profiling
  • Used for cytokine profiling in immune system disorders.
  • Supports research on autoimmune diseases and chronic inflammatory conditions.

Recommended Reconstitution & Handling

Reconstitution Protocol:

1. Reconstitute lyophilized M-CSF in 20 mM Tris-HCl, pH 8.0 to a concentration of 0.1–1.0 mg/mL.
2. Aliquot & Store at -20°C to -80°C for long-term use.
3. Avoid repeated freeze-thaw cycles to maintain protein activity.

Recommended Working Concentrations:

Storage & Handling Instructions

• Lyophilized Storage: Store at 2–8°C in a desiccated environment.
• Reconstituted Storage: Store at ≤-20°C to -80°C in working aliquots.
• Avoid repeated freeze-thaw cycles to prevent degradation.
• Use aseptic techniques when handling and diluting the protein.

Why Choose Gibco™ Human M-CSF Recombinant Protein?

✅ High bioactivity with ED50 ≤ 5 ng/mL for macrophage proliferation

✅ HEK293-expressed for superior stability and glycosylation

✅ Low endotoxin levels (<0.1 ng/µg) for consistent immunological studies

✅ Validated in functional assays including monocyte differentiation and VEGF secretion

✅ Versatile for immunology, stem cell, cancer, and drug discovery research

Gibco™ Human M-CSF Recombinant Protein is an essential cytokine for macrophage differentiation, immune modulation, and stem cell research, ensuring high-performance results in functional and cell-based assays.