Tagmentase (Tn5 transposase) - loaded

Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.

Tagmentase (Tn5 transposase) – loaded can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq.  Here below we are showing some examples of results.

Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase

ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).

Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase

ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.

Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase

ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).

Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase

Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).

Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase

Chromatin preparation and immunoprecipitation have been performed on 500,000 to 1,000,000 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K36me3 (Cat. No. C15410192), H3K27me3 (Cat. No. C15410195) and rabbit IgG (Cat. No. C15410206) have been used.