Dialysis tubing cellulose membrane, avg. flat width 43 mm (1.7 in.), 100ft.

This Sigma-Aldrich Dialysis Tubing is a high-quality cellulose membrane designed for efficient dialysis applications in laboratory settings. It has a typical molecular weight cut-off (MWCO) of 14,000, making it suitable for retaining proteins and molecules with molecular weights of 12,000 or greater. The tubing is provided in a dry roll format for convenience and ease of use.

Key Features:

1. Material and Dimensions:
   • Made from cellulose membrane.
   • Average flat width: 43 mm (1.7 inches).
   • Length: 100 ft (supplied in rolls).
2. Molecular Weight Cut-Off:
   • Typical MWCO: 14,000, effectively retaining most proteins and large biomolecules.
3. Supplied Quality:
   • May contain glycerin and sulfur compounds in more than trace amounts as part of the manufacturing process.
4. Preparation for Use:
   • Glycerin removal: Wash the tubing under running water for 3-4 hours.
   • Sulfur compound removal: Treat the tubing with a 0.3% sodium sulfide solution at 80°C for 1 minute. Follow with:
     • Hot water wash: 60°C for 2 minutes.
     • Acidification: Use a 0.2% sulfuric acid solution.
     • Final rinse: Rinse thoroughly with hot water to remove residual acid.
5. Applications:
   • Dialysis for protein purification, buffer exchange, and molecular separation.

Specifications:

Applications:

1. Protein Purification:
   • Ideal for separating proteins and peptides based on molecular size.
2. Buffer Exchange:
   • Used in desalting and removing small molecules like salts and solvents from biomolecular solutions.
3. Molecular Separation:
   • Effective for separating molecules in research involving nucleic acids, peptides, or other biomolecules.
4. Biotechnology and Research Labs:
   • Widely used in academic and industrial laboratories for routine dialysis applications.

Preparation Instructions:

• Removal of Glycerin:
  • Wash the tubing under running water for 3-4 hours to remove glycerol used as a humectant.
• Removal of Sulfur Compounds:
  • Treat with a 0.3% sodium sulfide solution at 80°C for 1 minute.
  • Rinse with hot water (60°C) for 2 minutes.
  • Acidify with a 0.2% sulfuric acid solution and rinse thoroughly with hot water.

Advantages:

• High Retention: Effectively retains molecules with molecular weights above 12,000.
• Customizable Preparation: Allows removal of manufacturing residues for tailored use.
• Long Length: The 100 ft roll format provides ample material for extended use.

Storage and Handling:

• Storage Condition: Keep dry in a clean environment to maintain membrane integrity.
• Handling: Prepare as per the provided instructions before use to ensure optimal performance.

The Sigma-Aldrich Dialysis Tubing Cellulose Membrane offers high reliability and flexibility for applications requiring molecular separation and protein purification. Its high MWCO and adaptability for laboratory protocols make it a trusted tool in molecular biology and biochemistry research.