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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₄₄H₅₅NO₁₆ |
Lipophilic, cell-retained fluorescent β-galactosidase substrate
Invitrogen™ C12FDG is a β-galactosidase substrate covalently modified with a 12-carbon lipophilic moiety to enhance cellular retention of the fluorescent product following enzymatic cleavage. Once inside the cell, β-galactosidase hydrolyzes C12FDG to release a green-fluorescent compound that is retained within the cytoplasm, likely due to integration of the lipophilic tail into the cell membrane.
C12FDG is the primary component of the ImaGene™ Green kit and is particularly useful in applications requiring long-term tracking of β-galactosidase activity in live cells, including flow cytometry, fluorescence microscopy, and gene expression studies involving LacZ reporters.
Key Features
- Lipophilic Modification – Enhances product retention within cells for extended signal detection
- Bright Green Fluorescence – Excitation/emission maxima ~490/514 nm
- Live-Cell Compatible – Suitable for in vitro β-galactosidase detection without significant cell toxicity
- High Specificity – Low background fluorescence, ideal for reporter assays
- Versatile Applications – Works with microscopy, plate readers, and flow cytometry platforms
Applications
- β-galactosidase reporter gene assays
- Long-term live-cell β-galactosidase detection
- LacZ expression analysis in mammalian cells
- Single-cell analysis by flow cytometry
- Monitoring of gene expression in transfection and viral transduction experiments
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | D2893 |
| Chemical Name | 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside |
| Quantity | 5 mg |
| Substrate Type | β-galactosidase substrate |
| Excitation/Emission | ~490/514 nm |
| Form | Solid |
| Detection Method | Fluorescence |
| Substrate Properties | Chemical substrate |
| Storage Conditions | –5°C to –30°C, protect from light |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ C12FDG provides sensitive, cell-retained detection of β-galactosidase activity for long-term live-cell imaging and flow cytometry applications. Its lipophilic modification ensures durable intracellular fluorescence, making it an ideal substrate for LacZ reporter assays in research requiring both sensitivity and retention.