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Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated)
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A non-selective pre-enrichment medium and is tested in accordance with ISO 65791 and ISO 11133:20142
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated) is a non-selective pre-enrichment medium designed to support the recovery of Salmonella spp. from food samples. Its formulation facilitates the resuscitation of sub-lethally injured cells, allowing them to repair and multiply before being introduced into selective media. Compliant with ISO 6579-1:2017 and ISO 11133:2014, this medium ensures reliability and consistency in food microbiology testing.
Key Features
- Supports High Recovery Rates:
- Rich in nutrients to promote intense growth and resuscitation of injured cells.
- Enhances Salmonella Recovery:
- The phosphate buffer system prevents pH fluctuations, aiding in the recovery of sub-lethally damaged cells.
- ISO Compliant:
- Tested and validated according to ISO 6579-1:2017 and ISO 11133:2014 standards, ensuring international compliance.
- Non-Selective Pre-Enrichment:
- Provides optimal conditions for Salmonella repair and multiplication without inhibiting bacterial growth.
- High Yield:
- One 500g package prepares up to 25.0L of medium, making it cost-effective for large-scale testing.
Applications
1. Food Microbiology
- Pre-enrichment of Salmonella spp. from:
- Raw and processed foods
- Meat and poultry
- Dairy products
- Fruits and vegetables
2. Food Chain Testing
- Pre-enrichment for ISO 6579-1:2017 workflows to ensure accurate detection and enumeration of Salmonella.
3. Regulatory and Compliance Testing
- Use in food safety testing workflows for regulatory compliance and quality assurance.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Pre-enrichment Medium |
| Form | Powder |
| Description | Buffered Peptone Water (ISO) |
| Buffer System | Phosphate buffer |
| Applications | Pre-enrichment of Salmonella spp. |
| Quantity | 500g |
| Yield | 25.0L of prepared medium |
| Certifications | ISO 6579-1:2017, ISO 11133:2014 |
Preparation Instructions
- Suspend: Suspend 20g of Buffered Peptone Water in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until completely dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool: Allow the medium to cool before inoculating with the sample.
Advantages
- Optimal Recovery of Salmonella spp.: Promotes repair and growth of sub-lethally damaged cells.
- Cost-Effective: High yield (25L per 500g) makes it ideal for routine and large-scale testing.
- Non-Selective Medium: Allows cells to multiply without inhibition, improving recovery chances.
- ISO Compliance: Ensures reliability in food microbiology workflows.
- Nutrient-Rich Formulation: Provides the necessary environment for effective pre-enrichment.
Diagnostic Features
| Component | Function |
|---|---|
| Phosphate Buffer | Maintains stable pH, preventing additional stress to Salmonella cells. |
| Peptone | Provides nutrients for cell recovery and growth. |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to the product label for expiration date.
Packaging
- Product: Buffered Peptone Water (ISO) (Dehydrated)
- Quantity: 500g
- Yield: 25.0L of prepared medium
The Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) is an essential tool for the pre-enrichment of Salmonella spp. in food microbiology workflows. Its nutrient-rich formulation, enhanced buffering capacity, and ISO compliance make it indispensable for food safety testing, enabling reliable recovery and detection of pathogens in various food matrices.