Thermo Scientific™ Oxoid™ Raka-Ray Agar Base (Dehydrated).

The Thermo Scientific™ Oxoid™ Raka-Ray Agar Base (Dehydrated) is a specialized medium designed for the isolation and enumeration of lactic acid bacteria in beer and brewing processes. Based on the formulation by Saha, Sondag, and Middlekauff, this medium is recommended by the American Society of Brewing Chemists (ASBC) and the European Brewing Convention (EBC) for detecting spoilage organisms such as Lactobacillus spp. and Pediococcus spp.. Raka-Ray Agar Base incorporates selective growth-stimulating agents to support the diverse nutritional requirements of lactic acid bacteria.

Key Features

1. Brewing Industry Standard:
   • Recommended by the ASBC and EBC for monitoring beer spoilage organisms.
2. Comprehensive Detection:
   • Supports the growth of a wide range of lactic acid bacteria, including Lactobacillus fructivorans and Pediococcus spp..
3. Optimized Formula:
   • Includes sorbitan mono-oleate, liver extract, yeast extract, and N-acetyl glucosamine to enhance colony size, number, and incubation time.
4. Selective Carbohydrate Sources:
   • Contains fructose for detecting Lactobacillus fructivorans and maltose for glucose-negative lactobacilli.
5. Reliable Performance:
   • Provides accurate monitoring of spoilage organisms, ensuring the quality of beer and brewing processes.

Applications

1. Brewing Microbiology

• Isolation and enumeration of lactic acid bacteria in beer and brewing materials.
• Detection of spoilage organisms such as Lactobacillus spp. and Pediococcus spp..

2. Quality Control

• Monitoring microbial contamination during brewing processes to ensure product quality.

3. Research and Development

• Studies on the role of lactic acid bacteria in beer spoilage and brewing microbiology.

Specifications

Preparation Instructions

1. Suspend: Suspend 77g of Raka-Ray Agar Base in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until fully dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Dispense: Pour the prepared medium into sterile Petri dishes or test tubes and allow it to solidify.

Advantages

1. Industry-Validated: Endorsed by leading brewing organizations (ASBC and EBC).
2. Enhanced Growth: Optimized formula ensures the recovery of diverse lactic acid bacteria.
3. Comprehensive Detection: Supports the detection of glucose-negative and fructose-positive lactobacilli.
4. Reliable Quality Control: Ensures accurate microbial monitoring in brewing processes.
5. Economical: High yield of 6.5L per 500g for routine laboratory use.

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to product label for expiration date.

Packaging

• Product: Raka-Ray Agar Base (Dehydrated)
• Quantity: 500g
• Yield: 6.5L of prepared medium

The Thermo Scientific™ Oxoid™ Raka-Ray Agar Base (Dehydrated) is a trusted solution for the detection and enumeration of lactic acid bacteria in beer and brewing processes. Its optimized formula, broad applicability, and industry endorsements make it an essential tool for quality control in the brewing industry.