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Thermo Scientific™ Oxoid™ Group B streptococci (GBS) Agar Base
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ GBS Agar Base (Islam) (Dehydrated) is a specialized medium designed for the isolation and detection of Group B streptococci (GBS) in clinical specimens. Based on the formulation described by Islam, this medium exploits the ability of most GBS strains to produce orange/red pigmented colonies under anaerobic incubation conditions, providing a clear and reliable method for detection.
Key Features
- Selective for Group B Streptococci:
- Designed to support the growth of GBS while suppressing non-target organisms.
- Pigment-Based Differentiation:
- GBS colonies appear orange/red, enabling easy differentiation and identification.
- Rapid and Reliable:
- Clear colonies, 0.5–1mm in diameter, are visible after 24–48 hours of anaerobic incubation.
- Broad Application:
- Effective for isolating GBS from clinical specimens, including CSF, blood, and other infected sites.
- Diagnostic Utility:
- Ideal for identifying GBS, a major cause of neonatal bacterial meningitis and infections in adults.
Applications
1. Clinical Microbiology
- Isolation and identification of Group B streptococci from:
- Neonatal specimens (e.g., CSF, blood).
- Maternal specimens to assess GBS colonization.
- Other clinical samples from infected adults.
2. Diagnostic Testing
- Detection of GBS in workflows for preventing neonatal infections caused by maternal transmission.
3. Research Microbiology
- Studies on GBS-related infections, including bacterial meningitis and adult infections.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | GBS Agar Base (Islam) |
| Applications | Clinical microbiology and diagnostics |
| Quantity | 500g |
| Yield | 11.1L of prepared medium |
Preparation Instructions
- Suspend: Suspend 45g of GBS Agar Base in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool and Dispense: Pour into sterile Petri dishes or tubes and allow to solidify.
Advantages
- Selective and Specific: Suppresses non-target organisms while supporting GBS growth.
- Easy Identification: Pigmented colonies provide clear visual differentiation of GBS.
- Diagnostic Reliability: Proven utility in detecting neonatal and adult infections caused by GBS.
- Cost-Effective: High yield of 11.1L per 500g, ideal for routine diagnostic workflows.
Diagnostic Features
| Organism | Colony Appearance |
|---|---|
| Group B Streptococci | Round, entire, orange/red colonies (0.5–1mm) |
| Non-Target Organisms | Growth suppressed or unpigmented colonies |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: GBS Agar Base (Islam) (Dehydrated)
- Catalog Number: CM0755B
- Quantity: 500g
- Yield: 11.1L of prepared medium
The Thermo Scientific™ Oxoid™ GBS Agar Base (Islam) is a reliable and efficient medium for isolating and detecting Group B streptococci in clinical samples. Its selectivity, pigment-based differentiation, and diagnostic reliability make it an essential tool for microbiology laboratories focused on neonatal, maternal, and adult GBS infections.