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Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated)
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examination of foodstuffs, detection of the Enterobacteriaceae that ferment glucose to produce acid
Specifications:
| Application | Cell culture, Tissue Culture, Microbiology, Clinical microbiology, Industrial Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | Target Organism Class | Enterobacteriaceae |
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated) is a selective medium formulated for the detection and enumeration of Enterobacteriaceae in food products. This glucose-containing medium enhances reproducibility in identifying typical colonies, making it a trusted tool for microbiological food safety testing. Its formulation is optimized for detecting glucose-fermenting Enterobacteriaceae, including coliforms, salmonellae, shigellae, and heat-resistant species such as Klebsiella and Citrobacter.
Key Features
- Selective for Enterobacteriaceae:
- Combines neutral red, crystal violet, and bile salts to inhibit Gram-positive bacteria and promote Enterobacteriaceae growth.
- Glucose Addition for Enhanced Detection:
- Glucose fermentation produces acid and/or gas, improving the identification of Enterobacteriaceae compared to lactose-only media.
- Easy-to-Interpret Results:
- Glucose fermentation lowers the pH, forming purple-pink colonies due to the neutral red indicator.
- ISO-Compliant:
- Developed to meet the recommendations of ISO standards, ensuring reproducibility and accurate counts for food safety.
Specifications
| Property | Details |
|---|---|
| Description | Violet Red Bile Glucose Agar (Dehydrated) |
| Form | Powder |
| Product Type | Agar |
| Quantity | 500 g |
| Yield | Prepares 13.0L of medium |
| Certifications | AFNOR, BSI, ISO, NMKL compliant |
| Catalog Number | CM0485B |
Applications
- Food Microbiology:
- Detect and enumerate Enterobacteriaceae in raw, processed, and heat-treated food products.
- Process Validation:
- Monitors heat-resistant species like Klebsiella and Citrobacter to identify processing failures.
- Environmental Monitoring:
- Tests for Enterobacteriaceae presence in food production facilities and handling environments.
- Research:
- Studies glucose fermentation and acid/gas production in Enterobacteriaceae.
Preparation Instructions
- Suspend 41.5g of Violet Red Bile Glucose Agar powder in 1 liter of distilled water.
- Heat gently to dissolve completely.
- Bring to a boil while stirring to prevent localized overheating.
- Do not autoclave. Cool to 45-50°C, pour into sterile Petri dishes, and allow to solidify.
Advantages
- Enhanced Detection: Glucose addition improves detection of lactose non-fermenting Enterobacteriaceae.
- Highly Selective: Crystal violet and bile salts inhibit Gram-positive bacteria, ensuring specificity.
- Accurate and Reliable: ISO-compliant formulation provides consistent and reproducible results.
- Broad Application: Suitable for food safety, process validation, and environmental monitoring.
Packaging
- Quantity: 500g of dehydrated powder.
- Yield: Prepares 13.0L of medium.
Why Choose Violet Red Bile Glucose Agar?
- A proven medium for food safety microbiology that adheres to international standards.
- Reliable detection of Enterobacteriaceae, including lactose non-fermenters.
- Backed by Thermo Scientific’s commitment to quality and innovation.