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Thermo Scientific™ Oxoid™ SIM Medium (Dehydrated)
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Differentiate enteric bacteria on the basis of sulfide production, indole production, and motility
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ SIM Medium (Dehydrated) is a multi-functional medium designed for the differentiation of enteric bacteria based on their ability to produce hydrogen sulfide (H₂S), indole, and their motility. This medium is particularly useful in identifying members of the Enterobacteriaceae family, such as differentiating Salmonella, Shigella, Klebsiella, Enterobacter, and Serratia species. Its semi-solid formulation facilitates the observation of bacterial motility.
Key Features
- Three-in-One Differentiation:
- Simultaneously tests for hydrogen sulfide production, indole production, and motility.
- Hydrogen Sulfide Detection:
- Contains sodium thiosulfate and ferrous ammonium sulfate as indicators for H₂S production, resulting in a black precipitate.
- Indole Detection:
- Includes tryptone, a tryptophan-rich peptone that is degraded by specific bacteria to produce indole.
- Motility Testing:
- Incorporates 0.35% agar, creating a semi-solid medium ideal for the examination of bacterial motility.
- Convenience and Reliability:
- Combines three diagnostic tests into one tube, simplifying workflows in clinical and food microbiology laboratories.
Typical Reactions in SIM Medium
| Organism | Motility | H₂S Production | Indole Production |
|---|---|---|---|
| Escherichia coli ATCC® 25922 | Variable | Negative | Positive |
| Proteus hauseri ATCC® 13315 | Positive | Positive | Positive |
| Shigella sonnei ATCC® 25931 | Negative | Negative | Negative |
Specifications
| Property | Details |
|---|---|
| Description | SIM Medium |
| Form | Powder |
| Product Type | Dehydrated Culture Media |
| Quantity | 500 g |
| Yield | Prepares 16.7L of medium |
| Certifications | FDA-compliant |
| Catalog Number | CM0435B |
Applications
- Clinical Microbiology:
- Differentiates members of the Enterobacteriaceae family in clinical specimens.
- Aids in the identification of pathogens such as Salmonella and Shigella.
- Food Microbiology:
- Identifies enteric bacteria in food and water samples for quality control.
- Research:
- Supports studies on bacterial motility, sulfur metabolism, and indole production.
Preparation Instructions
- Suspend 30g of SIM Medium powder in 1 liter of distilled water.
- Heat gently to dissolve completely.
- Dispense into test tubes.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Cool to room temperature, then inoculate as required.
Advantages
- Comprehensive Testing: Combines three critical diagnostic tests in a single medium, saving time and resources.
- Reliable Performance: Provides clear differentiation of enteric bacteria based on biochemical characteristics.
- Semi-Solid Formulation: Facilitates accurate motility testing.
- High Yield: Each 500g package prepares 16.7 liters of medium, offering excellent value for laboratories.
Packaging
- Quantity: 500g of dehydrated powder.
- Yield: Prepares 16.7L of medium.
Why Choose SIM Medium?
- Ideal for differentiating enteric bacteria with clear, interpretable results.
- Simplifies workflows with a single medium for three key tests.
- Trusted by microbiology laboratories for clinical, food, and environmental applications.