Oxoid Mrs Agar (De Man, Rogosa, Sharpe), 500G

The Thermo Scientific™ Oxoid™ MRS Agar (Dehydrated) is a solidified version of MRS Broth, specifically formulated for the cultivation of lactic acid bacteria. Developed by de Man, Rogosa, and Sharpe, MRS Agar provides a nutritionally rich medium that supports the growth of fastidious lactic acid bacteria, including Lactobacillus, Streptococcus, Pediococcus, and Leuconostoc species. It is ideal for applications requiring the selective isolation and enumeration of lactic acid bacteria in food and clinical samples.

Key Features

1. Optimized Formula:
   • Includes peptone, yeast extract, glucose, and essential salts to promote robust growth of lactic acid bacteria, including slow-growing strains.
2. Selective for Lactic Acid Bacteria:
   • Designed to inhibit competing microflora, supporting the growth of Lactobacillus spp. while tolerating lower pH levels.
3. Versatile Applications:
   • Suitable for food microbiology, dairy testing, and research on probiotic cultures.
4. Microaerophilic Conditions:
   • Facilitates the growth of lactic acid bacteria in anaerobic or microaerophilic environments.
5. Adjustable Selectivity:
   • Selectivity can be modified by adjusting pH or adding inhibitors like sorbic acid or thallous acetate.

Applications

1. Food Microbiology

• Enumeration and identification of lactic acid bacteria in:
  • Yogurt
  • Fermented foods
  • Dairy products
  • Beverages

2. Clinical Microbiology

• Cultivation of probiotic strains and identification of Lactobacillus spp. in clinical samples.

3. Research and Development

• Studies on lactic acid bacteria, including metabolic activity and probiotic functionality.

Specifications

Preparation Instructions

1. Suspend: Suspend 62g of MRS Agar powder in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until completely dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Dispense: Pour into sterile Petri dishes or tubes and allow to solidify.

Advantages

1. Selective and Differential: Inhibits competing organisms while supporting the growth of lactic acid bacteria.
2. Reproducible Results: Provides consistent results for enumeration and identification workflows.
3. Versatile Use: Applicable in food microbiology, clinical diagnostics, and probiotic research.
4. Cost-Effective: High yield of 8.1L per 500g, suitable for routine testing.

Incubation Techniques

• Incubate under anaerobic or microaerophilic conditions with 5% CO₂ to stimulate growth.

Quality Control

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C.
• Shelf Life: Refer to the product label for expiration date.
• Prepared Medium: Store at 2–8°C for short-term use.

Packaging

• Product: MRS Agar (Dehydrated)
• Quantity: 500g
• Yield: 8.1L of prepared medium

The Thermo Scientific™ Oxoid™ MRS Agar (Dehydrated) is a reliable and versatile medium for cultivating lactic acid bacteria in food, clinical, and research applications. Its optimized formula and ease of preparation make it an essential tool for microbiology laboratories focused on probiotic research, dairy testing, and fermented food analysis.