Thermo Scientific™ Oxoid™ Antibiotic Medium No.1 (Dehydrated)

The Thermo Scientific™ Oxoid™ Antibiotic Medium No.1 (Dehydrated) is a specialized microbiological medium designed for antibiotic assay work. Known for its enhanced formulation that eliminates inhibitory properties toward certain antibiotics, this medium is ideal for conducting microbiological assays of antibiotics using the disc diffusion or plate assay methods. It is particularly suited for antibiotics such as streptomycin, kanamycin, polymyxin B, and neomycin, ensuring reliable and reproducible results.

Antibiotic Medium No.1 is recommended for preparing the seed layer in the microbiological assay of antibiotics. It is compatible with various test organisms, including Micrococcus flavus, Sarcina lutea, and Staphylococcus aureus, for a wide range of antibiotic assays.

Key Features

1. Specially Modified Agar: Formulated using Oxoid Agar No.1, which lacks inhibitory properties toward specific antibiotics.
2. Wide Compatibility: Suitable for assays of multiple antibiotics, including kanamycin, penicillin G, methicillin, and oxacillin.
3. Reliable Results: Ensures consistent and reproducible results, even with antibiotics prone to interference.
4. Dehydrated Form: Easy to store and prepare; a single package yields up to 18.5L of medium.
5. Compliance Standards: Meets the requirements of AOAC, FDA, USDA, and ISO for industrial applications.

Applications

1. Antibiotic Assay Work

• Seed Layer Preparation: Used as the seed agar for plate assays with:
  • Micrococcus flavus for bacitracin assays.
  • Sarcina lutea for chloramphenicol assays.
  • Staphylococcus aureus for assays of kanamycin, penicillin G, methicillin, oxacillin, and vancomycin.

2. Base Agar for Antibiotic Assays

• Acts as a base layer for testing antibiotics such as:
  • Chloramphenicol
  • Kanamycin Sulphate
  • Colistin Sulphate
  • Sodium Methicillin
  • Sodium Oxacillin
  • Vancomycin Hydrochloride

3. Pharmaceutical Quality Control

• Widely used in the pharmaceutical industry to evaluate the potency and effectiveness of antibiotics.

4. Industrial and Regulatory Compliance Testing

• Supports compliance with regulatory standards for antibiotic potency assays as required by AOAC, FDA, USDA, and ISO.

Specifications

Preparation Instructions

1. Weigh the Medium: Suspend 27g of powder in 1 liter of distilled water.
2. Mix and Heat: Bring the mixture to a boil while stirring to ensure complete dissolution.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Cool and Pour: Cool the medium to 45–50°C, then pour into plates for solidification.

Advantages

1. Non-Inhibitory Properties: Ensures compatibility with antibiotics sensitive to agar components.
2. Versatile: Applicable for both seed layers and base layers in antibiotic assays.
3. Ease of Use: Dehydrated format allows for convenient preparation and long-term storage.
4. High Yield: A 500g package produces up to 18.5L of medium, making it cost-effective.
5. Regulatory Compliance: Suitable for use in regulated industries requiring strict assay validation.

Organisms and Antibiotics

Storage and Stability

• Storage: Keep in a cool, dry environment at 10–30°C in tightly sealed packaging.
• Shelf Life: Refer to the product label for the expiration date.

General References

• Hanus F. J., Sands J. G., and Bennett E. O. (1967). Applied Microbiology, 15(1): 31-34.

Packaging

• Product: Oxoid Antibiotic Medium No.1 (Dehydrated)
• Quantity: 500g
• Yield: Prepares 18.5L of medium

The Thermo Scientific™ Oxoid™ Antibiotic Medium No.1 (Dehydrated) is an essential medium for reliable and reproducible microbiological assays of antibiotics. Its non-inhibitory properties and compatibility with a wide range of antibiotics make it an invaluable tool for pharmaceutical quality control, clinical testing, and research laboratories. With its compliance to regulatory standards, this medium ensures accurate assay results for critical antibiotic evaluations.