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Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated)
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used for the detection of DNase activity in other organisms
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated) is a specialized medium used to detect deoxyribonuclease (DNase) enzyme activity in microorganisms, particularly Staphylococcus aureus. DNase enzymes catalyze the degradation of DNA by breaking phosphodiester linkages, creating clear zones around colonies that produce the enzyme in sufficient quantities. This medium is primarily used for the identification of Staphylococcus spp., but it is also applicable for other DNase-producing organisms, aiding in microbial differentiation and identification.
Key Features
- DNase Activity Detection:
- Allows clear visualization of DNA hydrolysis through clear zones around colonies.
- Staphylococcus Identification:
- Differentiates Staphylococcus aureus (DNase-positive) from Staphylococcus epidermidis and other Staphylococcus species.
- Broad Application:
- Useful in distinguishing Serratia marcescens (positive) from Klebsiella and Enterobacter (negative).
- Simple and Effective:
- Incorporates DNA in the agar, simplifying the detection of DNase activity.
- Reliable and Reproducible:
- Provides consistent results for microbiological workflows in clinical, food, and environmental testing.
Applications
1. Clinical Microbiology
- Identification and differentiation of Staphylococcus aureus from other Staphylococcus species.
- Differentiation of Serratia marcescens from Klebsiella-Enterobacter group.
2. Food Microbiology
- Detection of DNase-producing pathogens in food samples.
3. Research and Academic Studies
- Studies on DNase enzyme production in various microorganisms.
4. Environmental Microbiology
- Monitoring DNase-producing bacteria in environmental samples.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | DNASE Agar (Dehydrated) |
| Applications | Clinical, food, environmental, and research microbiology |
| Quantity | 500g |
Preparation Instructions
- Suspend: Suspend 42g of DNASE Agar in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool and Dispense: Pour the prepared medium into sterile Petri dishes and allow it to solidify.
Advantages
- Clear Visualization: Easily detects DNase activity through the formation of clear zones.
- Versatile Use: Applicable for identifying DNase activity in a variety of microorganisms.
- Reliable Differentiation: Differentiates key bacterial species based on DNase production.
- Cost-Effective: High yield and ease of preparation make it economical for routine use.
- Proven Methodology: Based on validated techniques for DNase detection.
Diagnostic Features
| Organism | Reaction |
|---|---|
| Staphylococcus aureus | Clear zone around colonies (positive) |
| Staphylococcus epidermidis | No clear zone (negative) |
| Serratia marcescens | Clear zone around colonies (positive) |
| Klebsiella-Enterobacter | No clear zone (negative) |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: DNASE Agar (Dehydrated)
- Quantity: 500g
The Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated) is an essential medium for detecting and identifying DNase-producing organisms, particularly in clinical and research microbiology. Its ability to differentiate Staphylococcus aureus, Serratia marcescens, and other microorganisms makes it an indispensable tool for microbiologists focused on diagnostics, food safety, and environmental monitoring.