Thermo Scientific™ Oxoid ™ Tryptone Soya Agar Ep/Usp/Jp/Bp (Dehydrated)

Thermo Scientific™ Oxoid™ Tryptone Soya Agar (TSA) is a general-purpose nutrient-rich medium suitable for the cultivation of a wide variety of microorganisms, including aerobes and anaerobes. It conforms to the standards of various international pharmacopoeias, making it suitable for pharmaceutical, clinical, and food microbiology applications.

Key Features:

1. Nutrient-Rich Composition:
   • Contains peptones derived from pancreatic and enzymatic digestion to provide essential amino acids and nutrients.
2. Wide Applicability:
   • Supports the growth of a variety of organisms in pharmaceutical, clinical, and food testing.
   • Compatible with the addition of sterile blood for the preparation of blood agar or chocolate agar.
3. Pharmacopoeia Compliance:
   • Conforms to EP, USP, JP, and BP standards, making it suitable for pharmaceutical testing.
4. Enhanced Performance:
   • Can be modified with supplements like lecithin and Polysorbate 80 for specific applications (e.g., microbial content testing of quaternary ammonium compounds).

Typical Formula (per liter):

Preparation Instructions:

1. Preparation of Dehydrated Medium:
   • Suspend 40 g of TSA powder in 1 liter of distilled water.
   • Heat to boiling with frequent stirring to dissolve completely.
   • Sterilize by autoclaving at 121°C for 15 minutes.
2. Addition of Blood for Blood Agar:
   • Cool the sterilized medium to 45–50°C.
   • Add 7% sterile blood and mix gently.
3. Chocolate Agar Preparation:
   • Heat blood-supplemented agar until the medium turns chocolate brown for optimal performance.

Applications:

1. General Cultivation:
   • Suitable for the growth of a wide range of aerobes and anaerobes.
2. Pharmaceutical Testing:
   • Growth promotion and suitability testing for microbial enumeration.
3. Food and Dairy Microbiology:
   • Use in bacterial counts on poultry and other food products.
4. Specialized Media:
   • Used as a base for blood agar, chocolate agar, and enhanced hemolysis agar (EHA).

Specifications:

Quality Control:

Non-Pharmaceutical Testing:

Pharmaceutical Testing:

Advantages:

Precautions:

• Hemolytic reactions on blood agar may vary with the type of blood used (e.g., horse vs. sheep blood).
• Handle the medium under aseptic conditions to avoid contamination.

This highly versatile, nutrient-rich medium is an essential tool for microbial cultivation in pharmaceutical, clinical, and food microbiology applications.