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Thermo Scientific™ Oxoid™ MRVP Agar (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ MRVP Medium (Dehydrated) is a glucose-phosphate medium designed for the Methyl-red (MR) and Voges-Proskauer (VP) tests, facilitating the differentiation of coli-aerogenes group organisms such as Escherichia coli and Enterobacter aerogenes. These tests detect specific metabolic byproducts, enabling laboratories to distinguish between organisms based on their carbohydrate metabolism pathways.
Key Features
- Dual Purpose Medium:
- Supports the Methyl-red (MR) test for acid production and the Voges-Proskauer (VP) test for acetylmethylcarbinol production.
- Glucose-Phosphate Composition:
- Promotes metabolic activity, ensuring clear and reproducible results.
- Coli-Aerogenes Differentiation:
- Differentiates Escherichia coli (MR-positive, VP-negative) from Enterobacter aerogenes (MR-negative, VP-positive).
- Validated by Historical Studies:
- Based on methodologies established by Clark & Lubs and Voges & Proskauer.
- Wide Application:
- Used in clinical, food, and water microbiology for differentiating lactose-fermenting coliforms.
Applications
1. Clinical Microbiology
- Differentiation of coliform organisms, particularly Escherichia coli and Enterobacter aerogenes, from clinical specimens.
2. Food Microbiology
- Identification of coliforms in food safety workflows, including dairy and processed foods.
3. Water Microbiology
- Detection of coliform contamination in drinking water supplies.
4. Research and Education
- Studies on bacterial carbohydrate metabolism and coliform group differentiation.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Form | Powder |
| Description | MRVP Medium (Clark and Lubs Medium) |
| Applications | Clinical, food, water, and research microbiology |
| Quantity | 500g |
| Yield | 29.4L of prepared medium |
Preparation Instructions
- Suspend: Suspend 17g of MRVP Medium powder in 1 liter of distilled water.
- Mix and Heat: Heat gently with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Dispense: Dispense into sterile tubes or bottles for testing.
Advantages
- Dual Functionality: Supports both MR and VP tests for comprehensive coliform differentiation.
- Validated Methodology: Based on established microbiological protocols for reliability.
- Versatile Use: Suitable for clinical, food, and water testing workflows.
- Cost-Effective: High yield of 29.4L per 500g, ideal for routine laboratory use.
Expected Test Results
| Organism | Methyl-Red Test | Voges-Proskauer Test |
|---|---|---|
| Escherichia coli | Red (Positive) | No color change (Negative) |
| Enterobacter aerogenes | Yellow (Negative) | Red (Positive) |
| Other coliforms | Variable | Variable |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: MRVP Medium (Dehydrated)
- Catalog Number: CM0043B
- Quantity: 500g
- Yield: 29.4L of prepared medium
The Thermo Scientific™ Oxoid™ MRVP Medium (Dehydrated) is a reliable and effective medium for performing the Methyl-red and Voges-Proskauer tests, enabling differentiation of the coli-aerogenes group based on carbohydrate metabolism. Its high yield, ease of preparation, and compatibility with clinical, food, and water microbiology workflows make it an indispensable tool for microbiology laboratories.