Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated)

Yeast Extract Agar is a versatile, nutrient-rich medium designed for the enumeration and cultivation of a broad spectrum of microorganisms, including bacteria, yeasts, and molds. It is widely used for the plate count of heterotrophic microorganisms in water samples, conforming to ISO 1133:2014 guidelines.

Key Features:

1. Rich Nutrient Profile:
   • Promotes the growth of bacteria, yeasts, and molds under mesophilic conditions.
2. ISO-Compliant:
   • Conforms to ISO 11133:2014, ensuring reliable performance for water microbiological testing.
3. Wide Application:
   • Supports the enumeration of aerobic mesophilic organisms at 22°C and 37°C, providing distinct microbial profiles.
4. Validated by References:
   • Follows the formula described by Windle Taylor for the examination of water supplies.

Specifications:

Applications:

1. Water Microbiology:
   • Used for the plate count of microorganisms in drinking water and other water supplies.
2. Heterotrophic Plate Counts:
   • Suitable for incubating at both 22°C (3 days) and 37°C (24 hours) for bacterial enumeration.
3. General Microbial Growth:
   • Cultivation of a wide range of bacteria, yeasts, and molds.

Preparation Instructions:

1. Reconstitution:
   • Dissolve the required amount of Yeast Extract Agar powder in distilled water as per the recommended yield.
2. Sterilization:
   • Sterilize by autoclaving at 121°C for 15 minutes.
3. Plating:
   • Pour the prepared medium into sterile Petri dishes and allow to solidify.
4. Incubation:
   • Use the prepared plates for enumeration or cultivation of microorganisms under specific incubation conditions.

Quality Assurance:

• Tested in accordance with ISO 11133:2014 for performance, preparation, and storage of culture media.
• Environment Agency Standards for drinking water microbiology (2012).

References:

1. Windle Taylor E. (1958). The Examination of Waters and Water Supplies, 7th ed., Churchill Ltd., London, pp. 394–398 and 778.
2. International Organization for Standardization. ISO 11133:2014. Microbiology of food, animal feed, and water—Preparation, production, storage, and performance testing of culture media.
3. Environment Agency (2012). The Microbiology of Drinking Water: Part 7—Methods for the Enumeration of Heterotrophic Bacteria.

Advantages:

Thermo Scientific™ Oxoid™ Yeast Extract Agar provides a dependable solution for microbial enumeration and cultivation, ensuring compliance with international water testing standards.