Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL

The Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit is an essential tool designed for calibrating the quantitative PCR (qPCR) instruments, including the QuantStudio™ 3 and QuantStudio™ 5 systems. This easy-to-use calibration kit allows researchers to establish the pure dye spectra and multicomponent values for up to 10 commonly used fluorescence dyes (FAM™, VIC™, ROX™, SYBR™, ABY™, JUN™, MUSTANG PURPLE™, TAMRA™, NED™, and Cy™5).

By using this kit, researchers can quickly and efficiently perform dye calibration on their 96-well PCR plates to ensure accurate and reproducible fluorescence detection for multiplex qPCR assays. The kit includes three separate 96-well plates, each containing specific dyes, allowing for simultaneous calibration of multiple dyes in a single run.

Key Features & Benefits

✅ Simultaneous Dye Calibration

• The 10-Dye Calibration Kit allows for simultaneous calibration of 10 different dyes, significantly reducing the time and effort required to perform individual dye calibrations.
• The kit contains three 96-well plates, each loaded with a different set of fluorescent dyes for accurate multicomponent calibration.

✅ Broad Dye Compatibility

• Compatible with a wide range of fluorescent dyes commonly used in real-time PCR, including:
  • FAM™, VIC™, ROX™, SYBR™ (for general use)
  • ABY™, JUN™, MUSTANG PURPLE™ (for multiplex assays)
  • TAMRA™, NED™, Cy™5 (for various applications in gene expression, SNP, and genotyping)

✅ Improved Multiplexing

• Dye formulations in Plate 2 are specifically designed to improve results with multiplexing assays, more accurately representing the fluorescent spectra of probes used in real-time PCR experiments.
• Enhances the sensitivity and accuracy of multiplex PCR assays by reducing spectral overlap between dyes.

✅ Designed for Flexibility and Speed

• The 96-well, 0.2 mL plates are ideal for high-throughput PCR assays, allowing for rapid and efficient calibration without the need for individual dye setups.
• Pre-configured plates minimize setup time and make it easier for users to perform the calibration on QuantStudio™ 3, 5, and 6 Pro/7 Pro PCR systems.

✅ Reliable Calibration Results

• Dye calibration ensures optimal instrument performance, providing accurate and reproducible fluorescence data.
• Essential for instrument maintenance and quality control, ensuring high-confidence results for gene expression, genotyping, and SNP detection.

Specifications

Applications

• Dye Calibration for Real-Time PCR Systems:
  • Ensures accurate fluorescence detection and signal optimization in multiplex qPCR assays.
• High-Throughput qPCR:
  • Ideal for large-scale qPCR experiments and gene expression studies involving multiple dyes.
• Instrument Performance Monitoring:
  • Provides a routine verification tool to monitor and maintain the performance of your QuantStudio™ or ViiA™ 7 systems.
• Multiplex PCR Assays:
  • Calibration helps achieve reliable results in multiplex assays by minimizing dye spectral overlap.

Why Choose the TaqMan™ 10-Dye Spectral Calibration Kit?

✅ Fast and Efficient Calibration: Perform 10-dye calibration in a single run, saving time in the lab.

✅ Improved Multiplex Performance: Ensures accurate results and optimized sensitivity for multiplex assays.

✅ Broad Dye Compatibility: Compatible with 10 commonly used dyes, including SYBR™, FAM™, ROX™, and Cy™5.

✅ Ensures Consistent Performance: Regular calibration ensures reliable PCR results in gene expression, genotyping, and mutation analysis.