Liofilchem Lactophenol Cotton Blue Droppers 10 bottles x 10 mL

The Liofilchem™ Lactophenol Cotton Blue Droppers are a blue staining solution used for the microscopic examination of fungi from clinical specimens, food, and environmental samples. The droppers allow for easy preparation of fungal slides, enabling clear visualization of fungal structures under a microscope.

Key Features

1. Rapid Staining:
   • Provides a delicate blue stain for clear observation of yeast cells, mycelia, and fruiting structures.
2. Ready-to-Use:
   • Convenient dropper bottles for accurate application.
3. Validated for Clinical and Laboratory Use:
   • Tested with control strains to ensure consistent performance.
4. Versatile Applications:
   • Suitable for clinical diagnostics, environmental testing, and food microbiology.

Kit Contents

• 10 Bottles of Lactophenol Cotton Blue Droppers (10 mL each).
• 1 Instruction Sheet.

Method Principle

• Staining Mechanism:
  • The methyl blue (Cotton Blue) binds to chitin in fungal cell walls, staining fungal structures blue, while the background appears pale blue for contrast.

Applications

1. Clinical Microbiology:
   • Examination of fungi in clinical specimens, such as skin, nails, and sputum.
2. Food and Environmental Microbiology:
   • Detection and identification of fungal contaminants in food and environmental samples.
3. Research:
   • Studies on fungal morphology and identification.

Test Procedure

1. Dispense a drop of Lactophenol Cotton Blue onto a clean microscope slide.
2. Use an inoculating needle to spread fungal culture into a thin layer on the slide.
3. Place a coverslip onto the preparation, avoiding air bubbles. Let it sit for 5 minutes.
4. Observe the slide under a microscope.

Interpretation of Results

• Result:
  • Fungal structures stain blue, making identification easier.

Composition (per 100 mL)

Storage and Shelf Life

• Storage:
  • Store at 2–8°C, protected from light.
  • Stable during transport at 18–25°C for 4 days or 35–39°C for 48 hours.
• Shelf Life:
  • Valid until the expiry date on the label.

Advantages

• Easy Application:
  • Dropper bottles provide precise and controlled dispensing.
• Rapid Visualization:
  • Stains fungal structures in just 5 minutes.
• Cost-Effective:
  • Each kit includes 10 bottles, suitable for multiple tests.
• Validated Performance:
  • Tested with standard fungal strains for reliability.

Warnings and Precautions

1. Classified as hazardous under current legislation; consult the Safety Data Sheet (SDS) before use.
2. For in vitro diagnostic use only.
3. Use in a professional laboratory setting with trained personnel.
4. Dispose of used materials in accordance with laboratory procedures and local regulations.

Limitations

• Stain is specific for fungal structures; further biochemical or molecular tests may be needed for species-level identification.

Packaging

References

1. Larone D.H. (1995). Medically Important Fungi: A Guide to Identification. ASM Press, Washington, D.C.
2. Lennette, E.H., et al. (1985). Manual of Clinical Microbiology, 4th ed. American Society for Microbiology.
3. Finegold, S.M., and Martin, W.J. (1982). Bailey and Scott’s Diagnostic Microbiology, 6th ed. The C.V. Mosby Co., St. Louis.

Why Choose Liofilchem Lactophenol Cotton Blue Droppers?

• Ease of Use: Dropper bottles simplify slide preparation.
• Proven Accuracy: Validated for clear fungal visualization.
• Versatile Application: Suitable for clinical, food, and environmental microbiology.