Thermo Scientific™ Imject™ Freund's Incomplete Adjuvant (FIA), 5 x 10 mL

Thermo Scientific™ Imject™ Freund's Incomplete Adjuvant (FIA) is a widely used immunological adjuvant that enhances immune responses to antigens. It is a water-in-oil emulsion that facilitates prolonged antigen exposure, increasing antibody production in research applications. FIA is typically used for booster injections following an initial immunization with Freund’s Complete Adjuvant (FCA), which contains Mycobacterium. Unlike FCA, FIA lacks Mycobacterium, reducing the risk of excessive inflammation and tissue necrosis.

This product is designed for research applications and is particularly valuable in antibody production, immunization studies, and vaccine development.

Key Features & Benefits

✔ Water-in-Oil Emulsion – Ensures slow antigen release, enhancing immune response.

✔ Mycobacterium-Free – Reduces the risk of excessive inflammation and tissue damage.

✔ Ideal for Booster Injections – Used for subsequent immunization rounds after an initial FCA injection.

✔ Prolonged Antigen Retention – Facilitates sustained immune activation and antibody production.

✔ Convenient Pre-Measured Ampules – Comes in 10 mL ampules for easy handling and reduced contamination risk.

✔ High Stability & Long Shelf Life – Ensures consistent performance for extended periods when stored at 4°C.

Applications

🔹 Antibody Production – Enhances immune response for monoclonal and polyclonal antibody development.

🔹 Vaccine Research – Used in preclinical vaccine trials to boost immune responses.

🔹 Immunization Studies – Supports experimental immunology and immune mechanism investigations.

🔹 Autoimmune Disease Research – Helps induce experimental immune system models for studying immune tolerance, chronic inflammation, and hypersensitivity.

🔹 Cancer Immunotherapy Studies – Used to stimulate immune responses in tumor antigen research.

🔹 Pathogen & Infection Research – Enhances immune response characterization against viral and bacterial infections.

Usage Guidelines

🔸 Initial Injection – Typically performed with Freund’s Complete Adjuvant (FCA) to induce a primary immune response.

🔸 Booster Injections – Freund’s Incomplete Adjuvant (FIA) is used in subsequent injections to enhance and maintain the immune response.

🔸 Antigen Preparation – The antigen must be homogenized thoroughly in the emulsion before injection.

🔸 Injection Route – Typically administered via subcutaneous (SC) or intradermal (ID) injections in laboratory animal models.

Storage & Stability

📦 Store at 4°C upon receipt.

❄ Do not freeze, as it may alter the composition and effectiveness of the emulsion.

⚠ Shelf Life: Long-term stability ensures usability over several months when stored correctly.

Safety Considerations

⚠ Freund’s Incomplete Adjuvant (FIA) should not be used in humans.

⚠ Proper biosafety precautions should be taken while handling.

⚠ Wear protective gloves and lab coats to prevent skin contact.

⚠ Avoid intramuscular (IM) injections in certain cases, as FIA can cause localized irritation.

⚠ Use ampule breakers when opening glass ampules to ensure safety.

Related Products

🔹 Imject™ Freund's Complete Adjuvant (FCA) – For primary immunization (contains Mycobacterium).

🔹 Imject™ Alum Adjuvant – Aluminum-based alternative adjuvant for enhanced humoral immune response.

Product Specifications

Thermo Scientific™ Imject™ Freund’s Incomplete Adjuvant (FIA) is a powerful and widely used immunological adjuvant designed to boost immune responses in preclinical research. Its mycobacterium-free formulation makes it ideal for booster injections, reducing the risk of excessive inflammation. FIA is essential for antibody production, vaccine research, autoimmune disease modeling, and cancer immunotherapy studies. Its convenient packaging and prolonged antigen retention ensure reliable and reproducible results in immunization protocols.