Proteintech™ Anti-Human CD21 Mouse IgG2a RecAb, 100 µg

The Proteintech™ Anti-Human CD21 Mouse IgG2a Recombinant Antibody (RecAb) is a high-quality monoclonal antibody specifically designed for the detection of CD21 (Complement Receptor 2, CR2) in human samples. This antibody is produced recombinantly to ensure superior consistency, specificity, and reproducibility across various experimental applications. It is suitable for use in flow cytometry, immunohistochemistry, western blotting, and other immunoassays.

Key Features:

1. Target Antigen:
   • Recognizes CD21, a cell surface receptor primarily expressed on mature B cells, follicular dendritic cells, and subsets of T cells. CD21 plays a critical role in immune regulation and complement activation.
2. Clone and Isotype:
   • Clone: Mouse IgG2a
   • Isotype: IgG2a, kappa
3. Recombinant Antibody:
   • Produced using recombinant DNA technology for high purity and lot-to-lot consistency.
   • Free from hybridoma-related variability.
4. Applications:
   • Validated for multiple applications, including:
     • Flow Cytometry (FCM): For cell surface staining.
     • Immunohistochemistry (IHC): For fixed tissue analysis.
     • Western Blotting (WB): For protein detection.
     • ELISA: For quantitative detection of CD21.
5. Specificity and Sensitivity:
   • Exhibits high specificity for human CD21 with minimal cross-reactivity to other proteins.
6. Host:
   • Recombinantly produced from mouse cells, ensuring reliable and reproducible results.
7. Formulation:
   • Supplied as a liquid in a stabilized buffer to preserve activity and prolong shelf life.
   • Buffer contains PBS, with 0.02% sodium azide as a preservative.
8. Storage:
   • Store at 2–8°C for short-term use.
   • For long-term storage, aliquot and freeze at -20°C or lower to avoid repeated freeze-thaw cycles.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect and quantify CD21 expression on cell surfaces, such as B cells and follicular dendritic cells.
2. Immunohistochemistry:
   • Analyze CD21 expression in fixed tissue sections to study lymphoid organ architecture and B-cell localization.
3. Western Blotting:
   • Confirm the presence of CD21 protein in lysates of human cells or tissues.
4. ELISA:
   • Quantify CD21 in biological samples for research purposes.

Key Benefits:

1. Reproducibility:
   • Recombinant production ensures batch-to-batch consistency for reliable results across experiments.
2. Specificity:
   • Designed for high specificity toward human CD21, reducing background noise.
3. Flexibility:
   • Validated for multiple applications, making it a versatile reagent for immunological studies.
4. Stability:
   • Stabilized formulation ensures long shelf life and reliable performance over time.
5. Ease of Use:
   • Supplied ready-to-use, with clear guidance for application protocols.

Storage and Handling:

• Store at 2–8°C for immediate use or aliquot and store at -20°C or lower for long-term preservation.
• Avoid repeated freeze-thaw cycles to maintain antibody activity.

Research Use Only:

This product is intended for research purposes only. It is not for diagnostic or therapeutic use in humans or animals.