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Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene™ Blood RNA Tubes, 96 Preps
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is designed for high-yield, high-purity total RNA isolation (including miRNA) from PAXgene™ Blood RNA Tubes, using a magnetic bead-based workflow. This kit is optimized for low- to high-throughput applications and includes a TURBO DNase™ step to effectively eliminate contaminating genomic DNA.
With no spin columns required, this kit simplifies RNA purification and enables consistent, high-quality results suitable for real-time RT-PCR, qPCR, microarray, NGS, and other sensitive downstream applications.
Key Features and Benefits
- Compatible with PAXgene™ Blood RNA Tubes: Ideal for use with stabilized whole blood samples
- Total RNA Recovery: Isolates both large RNA and miRNA in a single protocol
- High Purity: DNase treatment removes gDNA, ensuring RNA is free of PCR inhibitors
- Flexible Formats: Use in microfuge tubes, manual or automated 96-well plates
- Efficient Workflow: Magnetic bead technology reduces hands-on time and improves reproducibility
- High Yield: Recover 3–18 µg of total RNA per 2.5 mL blood sample
- miRNA Compatibility: Co-purifies microRNAs without the need for a separate protocol
Applications
- Gene expression profiling (qPCR, RT-PCR)
- Transcriptome analysis (RNA-Seq, microarrays)
- miRNA analysis
- cDNA library construction
- Northern blotting, in vitro translation, and labeling
- Nuclease protection and hybridization assays
Performance Specifications
| Parameter | Specification |
|---|---|
| Sample Type | Whole blood (PAXgene™ tubes) |
| Yield (Total RNA) | 3–18 µg |
| miRNA Recovery | Yes (co-isolated) |
| A260/280 Ratio | 1.8–2.2 |
| RIN Value | ≥ 8 |
| gDNA Contamination | ≤ 1.56% |
| Purification Time | < 2 hours (manual); faster with automation |
| Elution Volume | 20–80 µL |
| Throughput | Low, medium, and high-throughput compatible |
Kit Contents & Storage
Box 1 (Room Temperature / 4°C)
- RNA Binding Beads (2 mL) – 4°C
- Resuspension Solution (18 mL)
- Wash Solution 1 (16 mL, concentrated)
- Wash Solution 2 (2 × 30 mL, concentrated)
- RNA Rebinding Buffer (10 mL)
- Elution Buffer (10 mL)
- Processing Plate (1)
- Tubes (100)
Box 2 (Frozen, -20°C)
- Proteinase (0.6 mL)
- TURBO DNase (1 mL)
- 10X TURBO DNase Buffer (1 mL)
Note: Magnetic stands and PAXgene™ tubes are sold separately.
Equipment Required
- Refrigerated centrifuge with 50 mL conical tube adapters
- Magnetic stand (e.g., MagMAX™ Express-96 processor or manual 96-well magnet)
- Microcentrifuge (for tube-based workflows)
- Multichannel pipette (for 96-well workflows)
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit offers a high-efficiency, scalable, and automation-ready solution for extracting high-quality total RNA and miRNA from stabilized whole blood samples. With exceptional purity, yield, and ease of use, it is the ideal RNA isolation solution for transcriptomic and molecular research workflows using PAXgene™ Blood RNA Tubes.