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Thermo Scientific™ Invitrogen™ eBioscience™ CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine7
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD184 (CXCR4) Monoclonal Antibody (Clone 12G5), PE-Cyanine7 is a fluorescently conjugated antibody designed for flow cytometry-based detection of human CXCR4 (CD184). CXCR4 is a chemokine receptor involved in immune cell trafficking, stem cell homing, viral entry (notably HIV), and tumor metastasis. This antibody enables accurate, sensitive, and high-resolution detection of CXCR4-expressing cells.
Conjugated to PE-Cyanine7, a bright tandem dye with high emission at ~775 nm, this antibody is optimized for multicolor flow cytometry and provides strong signal intensity and minimal spillover.
Key Features
- High specificity for human CD184 (CXCR4), validated in multiple studies
- Clone 12G5, a widely referenced tool in immunology, virology, and oncology research
- PE-Cyanine7 conjugation for optimal brightness in multicolor flow cytometry
- Pre-titrated for convenient use (5 µL/test)
- Affinity-purified and filtered for purity and safety
- Cross-adsorbed to minimize non-specific binding
Applications
- Flow Cytometry – validated for use in staining human peripheral blood lymphocytes (PBLs)
- HIV receptor expression studies
- Stem cell and hematopoietic progenitor analysis
- Tumor immunophenotyping
- Chemokine receptor signaling assays
Excitation and Emission
| Laser Line | Excitation Max | Emission Max |
|---|---|---|
| Blue/Green/Yellow-Green | ~569 nm | ~775–780 nm |
Specifications
| Attribute | Value |
|---|---|
| Target Protein | CD184 (CXCR4) |
| Clone | 12G5 |
| Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, κ |
| Conjugate | PE-Cyanine7 |
| Form | Liquid |
| Volume per Test | 5 µL (0.25 µg) |
| Buffer | PBS, pH 7.2 + BSA + 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light. Do not freeze. |
| Shipping | Ambient (domestic), Wet Ice (international) |
| RRID | AB_1659706 |
Product-Specific Information
- Highly cited in >20 peer-reviewed publications
- Can be combined with other fluorophores in multicolor panels
- Antibody binding partially inhibits SDF-1-induced CXCR4 signaling
- Blocks HIV-1/HIV-2 entry in vitro
- Compatible with fixation using IC Fixation Buffer or Fix/Lyse Solutions
Target Information: CXCR4 (CD184)
CXCR4 is a G-protein-coupled receptor (GPCR) for the chemokine CXCL12 (SDF-1) and is involved in immune cell homing, hematopoiesis, and CNS development. It acts as a coreceptor for HIV-1 and HIV-2 and is implicated in cancer progression, metastasis, and angiogenesis. The receptor is expressed on naive T cells, B cells, monocytes, and various stem and progenitor cells.
The CD184 (CXCR4) PE-Cyanine7 Antibody (12G5 clone) is a powerful and validated tool for researchers investigating chemokine signaling, immune function, HIV entry, or tumor immunobiology. Its bright, reliable PE-Cy7 conjugate ensures accurate and reproducible flow cytometric analysis, even in complex multicolor panels.