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Thermo Scientific™ Pierce™ IgG Elution Buffer, pH 2.0
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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ IgG Elution Buffer (pH 2.0) is a low-pH, ready-to-use elution buffer specifically formulated for the efficient dissociation of IgG antibodies from affinity resins such as Protein A, Protein G, and Protein L agarose supports. The acidic pH facilitates rapid and complete antibody elution without compromising structural integrity when promptly neutralized after collection.
This formulation is part of the Pierce™ series of high-yield, nondenaturing antibody purification buffers and is ideal for both immunoglobulin purification and protein-protein interaction studies where acidic elution is required for dissociation of bound complexes.
Key Features & Benefits
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Effective Low-pH Elution (pH 2.0)
Facilitates strong elution of IgG from Protein A, G, and L resins while maintaining antibody structure. -
Ready-to-Use Convenience
Pre-formulated and prefiltered 1X solution for immediate use in purification workflows. -
Compatible with Multiple IgG-Binding Supports
Works effectively with Protein A, G, A/G, and L affinity resins. -
Suitable for Antibody-Antigen Complex Dissociation
Enables recovery of antibodies or target proteins in protein interaction assays. -
Reliable and Reproducible
Manufactured to high quality standards for consistent results across experiments.
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Pierce™ IgG Elution Buffer |
| pH | 2.0 |
| Form | Liquid (1X solution) |
| Volume | 1 L |
| Application | Elution of antibodies from affinity columns |
| Compatibility | Protein A, Protein G, Protein L |
| Solution Type | Elution Buffer |
| Storage Conditions | Store at 4°C |
| Product Line | Pierce™ |
| Intended Use | For Research Use Only (RUO) |
Applications
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Antibody Purification Workflows
Efficiently elutes IgG from Protein A, G, A/G, and L affinity resins after sample loading and washing. -
Immunoprecipitation (IP) & Co-IP
Recovers immunoprecipitated antibodies or antigen complexes in acidic conditions. -
Protein-Protein Interaction Studies
Dissociates interacting proteins from affinity matrices for downstream analysis. -
Proteomics & Antibody Characterization
Supports workflows requiring recovery of antibodies or target proteins for SDS-PAGE, Western blotting, or mass spectrometry.
Thermo Scientific™ Pierce™ IgG Elution Buffer, pH 2.0 is a powerful, easy-to-use solution for affinity-based antibody purification and protein complex dissociation. Its low-pH formulation ensures high recovery yields and compatibility with a wide range of affinity resins, making it a dependable component for any antibody purification or protein interaction workflow.