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Invitrogen™ Novex™ ECL Chemiluminescent Substrate Reagent Kit
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Reagent A (125 ml)
Reagent B (125 ml)
Specifications:
| Application | Western Blot | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Novex™ ECL Chemiluminescent Substrate Reagent Kit is a highly sensitive, non-radioactive detection system designed for Western blot and dot blot applications utilizing horseradish peroxidase (HRP)-conjugated antibodies. This kit is part of the trusted Novex™ product line from Invitrogen™ and provides a robust and efficient method for chemiluminescence-based immunodetection.
Product Description
The Novex™ ECL kit comprises a two-component system:
- Reagent A: Luminol, the primary chemiluminescent substrate.
- Reagent B: An enhancer solution that boosts light emission intensity.
When combined in equal volumes and applied to a blot membrane with HRP-labeled antibodies, the reagents produce intense and sustained light emission, enabling sensitive detection of protein targets using X-ray film or CCD imaging systems.
Key Features
- High Sensitivity: Detects low picogram to nanogram levels of target proteins.
- Enhanced Signal Intensity: Strong chemiluminescent signal due to optimized luminol and enhancer formulation.
- Easy to Use: Two-part system mixed in equal parts at the time of use.
- Stable and Consistent: Reliable performance across multiple Western blot runs.
- Flexible Detection: Compatible with both film and digital imaging platforms.
Applications
- Western blotting using HRP-labeled primary or secondary antibodies
- Dot blot assays for protein and antigen detection
- Ideal for use in routine protein expression validation, signal transduction research, and immunological assays
Product Specifications
| Attribute | Description |
|---|---|
| Catalog Number | WP20005 |
| Reagent Type | Chemiluminescent Substrate |
| Target Enzyme | HRP (Horseradish Peroxidase) |
| Substrate | Luminol |
| Detection Method | Chemiluminescence |
| Form | Liquid |
| Quantity | 2 × 125 mL (Reagent A and B) |
| Shipping Condition | Room Temperature or Wet Ice |
| Storage Condition | Store at 4°C |
| Unit Size | Each kit includes two 125 mL bottles |
Kit Contents
- 125 mL Reagent A (Luminol)
- 125 mL Reagent B (Enhancer)
- Ready for immediate use by mixing 1:1 before application
The Novex™ ECL Chemiluminescent Substrate Kit provides a dependable and sensitive method for detecting HRP-conjugated proteins on blot membranes. Whether you're validating protein expression or probing for low-abundance targets, this kit delivers strong signal intensity, low background, and consistent results, making it an essential tool for your protein detection workflows.