QIAGEN QIAcard® FTA® CloneSaver Indicating cards, 96 Sample areas per card

The QIAcard FTA CloneSaver is a high-throughput, filter-based DNA archiving and purification system designed for the long-term storage and recovery of bacterial clones. Utilizing proprietary FTA technology, this innovative platform enables laboratories to preserve genetic material at ambient temperature, eliminating the need for costly and space-intensive –80°C freezer storage.

Engineered in a 96-well format, CloneSaver streamlines clone handling, archiving, and downstream processing, making it ideal for high-throughput molecular biology, genomics, and biotechnology workflows. The system not only preserves DNA integrity for extended periods but also protects samples from phage contamination, ensuring reliable and reproducible results.

Key Features

High-Throughput 96-Well Format

• Designed for parallel processing of multiple clones
• Ideal for high-throughput screening and archiving workflows
• Simplifies sample organization and tracking

Long-Term Ambient Storage

• Enables stable DNA preservation at room temperature
• Eliminates reliance on freezers and cold-chain logistics
• Reduces operational costs and energy consumption

FTA Technology for DNA Protection

• Chemically treated matrix lyses cells and stabilizes nucleic acids
• Protects stored clones from phage infection and degradation
• Ensures long-term integrity of genetic material

Direct DNA Recovery & Purification

• Allows simple punch-based recovery of DNA
• DNA can be used directly in downstream applications
• Minimizes sample processing steps and time

Broad Downstream Compatibility

• Suitable for:
  • PCR amplification
  • Electroporation
  • Heat-shock transformation
• Supports cloning, sequencing, and genetic analysis workflows

Applications

• High-throughput clone archiving and storage
• Bacterial and plasmid DNA preservation
• Genomic and molecular biology research
• PCR-based clone screening and amplification
• Synthetic biology and genetic engineering
• Transformation workflows (electroporation & heat-shock)
• Biopharmaceutical and biotechnology research

Technical Specifications

Workflow Integration

Clone Culture → Application onto CloneSaver → Drying → Ambient Storage → Punch Extraction → PCR / Transformation / Sequencing

This workflow enables:

• Efficient clone preservation without freezing
• Rapid DNA recovery for downstream applications
• Reduced handling and processing time

Usage Guidelines

1. Apply bacterial clones directly onto the CloneSaver card wells
2. Allow samples to dry completely at room temperature
3. Store in a dry, contamination-free environment
4. Recover DNA using a standard punch-out method
5. Use recovered material directly for PCR or transformation workflows

Unique Advantages

• Replaces –80°C freezer storage with ambient DNA archiving
• Enables high-throughput clone management in a compact format
• Provides built-in protection against phage contamination
• Reduces laboratory costs and simplifies logistics
• Supports direct downstream applications without additional purification
• Ideal for large-scale genomics and synthetic biology workflows

Regulatory & Safety Information

• For Research Use Only (RUO) – Not intended for diagnostic or therapeutic use
• Handle biological samples using appropriate biosafety practices
• Store in a dry, clean environment to maintain sample integrity

The QIAcard FTA CloneSaver is a powerful, cost-effective solution for modern laboratories seeking efficient, high-throughput clone storage and DNA purification. By combining FTA-based preservation, ambient storage, and direct downstream compatibility, it offers a streamlined alternative to traditional freezer-based systems—making it an essential tool for genomics, molecular biology, and biotechnology research.