Specifications:
Application | Next Generation Sequencing, NGS Library Preparation |
Storage Temperature | -20°C |
Product Type | NGS library Prep kits |
Product Brand | Hologic Diagenode |
Product Grade | Molecular Biology |
The UDI (Unique Dual Index) for Tagmented Libraries – Set I provides high-quality indexing primers designed for multiplexing up to 8 or 24 samples for sequencing on Illumina platforms. These validated UDI primer pairs enhance library multiplexing efficiency and minimize index hopping, ensuring accurate and reliable sequencing results.
The UDI for Tagmented Libraries – Set I is specifically designed for tagmentation-based library preparation workflows, including:
- μChIPmentation for Histones (Cat. No. C01011011)
- ChIPmentation Kit for Histones (Cat. No. C01011009)
- TAG Kit for ChIPmentation (Cat. No. C01011030)
- ATAC-seq Kit (Cat. No. C01080002)
Additionally, the 24 UDI set is compatible with other tagmentation-based library preparation methods, such as CUT&Tag sequencing.
Key Features & Benefits
1. High-Throughput Sample Multiplexing
- Enables multiplexing of up to 8 or 24 samples per set for Illumina sequencing.
- All three UDI sets (Set I, Set II, and Set III) can be used simultaneously for multiplexing up to 72 samples.
2. Minimized Index Hopping & Enhanced Accuracy
- Unique dual-indexing (UDI) strategy reduces misassignment of sequencing reads.
- Ensures reliable and accurate sequencing data by mitigating cross-contamination.
3. Broad Compatibility with Tagmentation-Based Workflows
- Designed for ChIPmentation, ATAC-seq, and CUT&Tag applications.
- Compatible with various tagmentation-based sequencing library preparation protocols.
Available UDI Sets & Ordering Information
Catalog Number | Description | Multiplexing Capacity |
---|---|---|
C01011034 | 24 UDI for Tagmented Libraries – Set I | Up to 24 samples |
C01011036 | 24 UDI for Tagmented Libraries – Set II | Up to 24 samples |
C01011037 | 24 UDI for Tagmented Libraries – Set III | Up to 24 samples |
- All three sets can be combined for a total multiplexing capacity of up to 72 samples.
Use Cases & Applications
1. Epigenetics & ChIP Sequencing
- Optimized for ChIPmentation-based histone profiling.
- Ensures accurate indexing in chromatin immunoprecipitation (ChIP) workflows.
2. ATAC-seq & Chromatin Accessibility Studies
- Enables multiplexed sequencing in ATAC-seq workflows, reducing per-sample costs.
- Provides high-quality index sequences for chromatin accessibility studies.
3. CUT&Tag & Single-Cell Genomics
- Compatible with CUT&Tag-based library preparation, offering unique dual indexes for precise data analysis.
- Facilitates multiplexed single-cell sequencing experiments.
The UDI for Tagmented Libraries – Set I provides unique dual-index primer pairs for tagmentation-based sequencing workflows, ensuring high-throughput multiplexing, reduced index hopping, and compatibility with Illumina platforms. Available in 8 or 24 UDI configurations, this indexing solution is ideal for ChIPmentation, ATAC-seq, and CUT&Tag sequencing applications.
- Pack Size: 24 UDI for 24 Samples 8 UDI for 8 Samples