24 UDI for Tagmented libraries - Set I

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Specifications:
Application Next Generation Sequencing, NGS Library Preparation
Storage Temperature -20°C
Product Type NGS library Prep kits
Product Brand Hologic Diagenode
Product Grade Molecular Biology

The UDI (Unique Dual Index) for Tagmented Libraries – Set I provides high-quality indexing primers designed for multiplexing up to 8 or 24 samples for sequencing on Illumina platforms. These validated UDI primer pairs enhance library multiplexing efficiency and minimize index hopping, ensuring accurate and reliable sequencing results.

The UDI for Tagmented Libraries – Set I is specifically designed for tagmentation-based library preparation workflows, including:

  • μChIPmentation for Histones (Cat. No. C01011011)
  • ChIPmentation Kit for Histones (Cat. No. C01011009)
  • TAG Kit for ChIPmentation (Cat. No. C01011030)
  • ATAC-seq Kit (Cat. No. C01080002)

Additionally, the 24 UDI set is compatible with other tagmentation-based library preparation methods, such as CUT&Tag sequencing.

Key Features & Benefits

1. High-Throughput Sample Multiplexing

  • Enables multiplexing of up to 8 or 24 samples per set for Illumina sequencing.
  • All three UDI sets (Set I, Set II, and Set III) can be used simultaneously for multiplexing up to 72 samples.

2. Minimized Index Hopping & Enhanced Accuracy

  • Unique dual-indexing (UDI) strategy reduces misassignment of sequencing reads.
  • Ensures reliable and accurate sequencing data by mitigating cross-contamination.

3. Broad Compatibility with Tagmentation-Based Workflows

  • Designed for ChIPmentation, ATAC-seq, and CUT&Tag applications.
  • Compatible with various tagmentation-based sequencing library preparation protocols.

Available UDI Sets & Ordering Information

Catalog NumberDescriptionMultiplexing Capacity
C0101103424 UDI for Tagmented Libraries – Set IUp to 24 samples
C0101103624 UDI for Tagmented Libraries – Set IIUp to 24 samples
C0101103724 UDI for Tagmented Libraries – Set IIIUp to 24 samples
  • All three sets can be combined for a total multiplexing capacity of up to 72 samples.

Use Cases & Applications

1. Epigenetics & ChIP Sequencing

  • Optimized for ChIPmentation-based histone profiling.
  • Ensures accurate indexing in chromatin immunoprecipitation (ChIP) workflows.

2. ATAC-seq & Chromatin Accessibility Studies

  • Enables multiplexed sequencing in ATAC-seq workflows, reducing per-sample costs.
  • Provides high-quality index sequences for chromatin accessibility studies.

3. CUT&Tag & Single-Cell Genomics

  • Compatible with CUT&Tag-based library preparation, offering unique dual indexes for precise data analysis.
  • Facilitates multiplexed single-cell sequencing experiments.

The UDI for Tagmented Libraries – Set I provides unique dual-index primer pairs for tagmentation-based sequencing workflows, ensuring high-throughput multiplexing, reduced index hopping, and compatibility with Illumina platforms. Available in 8 or 24 UDI configurations, this indexing solution is ideal for ChIPmentation, ATAC-seq, and CUT&Tag sequencing applications.

  • Pack Size:   24 UDI for 24 Samples 8 UDI for 8 Samples

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