Thermo Scientific™ Pierce™ Alkylating Reagents

Thermo Scientific™ Pierce™ Alkylating Reagents are supplied in convenient single-use, pre-weighed vials, streamlining protein alkylation workflows and minimizing handling errors. These reagents are designed for sulfhydryl group alkylation, commonly used in proteomics, mass spectrometry, and peptide mapping to prevent disulfide bond reformation by blocking reduced cysteine residues.

This no-weigh format eliminates the risk of moisture absorption, oxidation, and cross-contamination associated with repeatedly opening bulk containers. Each vial contains a fresh, precisely measured quantity of reagent, ensuring consistent reactivity and reproducibility across experiments.

Key Features & Benefits

• Single-Use, Pre-Weighed Vials
  Ready-to-use format eliminates weighing errors and contamination risk.
• High Purity Iodoacetamide and Chloroacetamide
  Optimized for protein alkylation post-reduction in proteomics workflows.
• Prevents Disulfide Bond Reformation
  Covalently modifies cysteines with a carbamidomethyl group (+57.07 Da).
• Enhanced Reproducibility
  Standardized reagent amounts support consistent mass spec and protein analysis results.
• Protects Reagent Stability
  Avoids degradation from repeated vial opening or environmental exposure.

Chemical & Application Information

Mechanism of Action

• Alkylation Target: Reduced cysteine (–SH) groups
• Chemical Reaction: Covalent modification with iodoacetamide or chloroacetamide
• Mass Addition: Carbamidomethyl group (+57.07 Da)
• Downstream Benefits: Maintains protein conformation; improves MS/MS data quality

Note: Excess iodoacetamide can react with reducing agents if added post-alkylation. Use immediately after cysteine reduction.

Applications

• Proteomics Sample Prep
  • Prevents disulfide bond reformation post-reduction
  • Ensures peptide integrity for LC-MS/MS
• Peptide Mapping & Mass Spectrometry
  • Enhances fragmentation pattern clarity
  • Improves mass accuracy in cysteine-containing peptides
• Enzyme Kinetics & Structural Biology
  • Preserves active site cysteines in reduced state
  • Maintains protein structure in solution

Thermo Scientific™ Pierce™ Alkylating Reagents (Iodoacetamide and Chloroacetamide) offer a convenient, contamination-free solution for blocking reduced cysteines during protein and peptide analysis workflows. With ready-to-use, single-use packaging, these reagents improve consistency, accuracy, and efficiency in proteomics, mass spectrometry, and biochemical research.