Thermo Scientific™ imidazole Buffer solution, 0.5 M

Thermo Scientific™ Imidazole Buffers are high-quality, ready-to-use 0.5 M aqueous solutions of imidazole, specifically formulated for biochemical and molecular biology workflows. Available in pH values of 6.5, 7.0, and 7.5, these buffers are ideal for applications such as protein purification via immobilized metal affinity chromatography (IMAC), where precise pH and concentration are critical to elution efficiency and protein integrity.

Imidazole functions both as a buffering agent and as a competitive ligand in affinity purification, particularly in the context of His-tagged protein elution. The buffers are supplied in clear, colorless liquid form and packaged in inert glass bottles to ensure chemical stability and purity. Their 0.5 M concentration provides robust buffering capacity for consistent and reproducible results.

Key Features

• 0.5 M Concentration: Provides strong and stable buffering across the physiological pH range.
• Defined pH Options: Available in pH 6.5, 7.0, and 7.5 to suit a variety of purification protocols.
• Pre-made & Ready-to-Use: Saves time and eliminates errors associated with buffer preparation.
• High Purity: Manufactured under controlled conditions to ensure batch-to-batch consistency.
• Glass Packaging: Protects buffer from contaminants and leachables; ideal for sensitive biochemical applications.
• Refrigerated Storage: Maintained between 2–8 °C to preserve stability and shelf life.

Applications

• Affinity Chromatography: Elution buffer for His-tagged proteins in IMAC protocols.
• Enzymatic Assays: Maintains pH stability for optimal enzyme activity.
• Protein Crystallization: Used as part of crystallization buffers in structural biology.
• Molecular Biology Protocols: Suitable for sample preparation, purification, and downstream analysis involving nucleic acids and proteins.

Available Formats

Specifications

• CAS Number: 288-32-4
• MDL Number: MFCD00005183
• Physical Form: Liquid
• Appearance: Colorless
• Storage Temperature: 2–8 °C
• Buffer Type: Imidazole, 0.5 M aqueous solution

Thermo Scientific™ 0.5 M Imidazole Buffers provide a reliable, convenient, and high-performance solution for researchers working in protein purification and other life science applications. With accurate pH adjustment and robust buffering strength, these solutions streamline workflows and ensure consistency across experiments. Whether used in chromatography, enzyme reactions, or structural studies, these buffers are an essential component of any molecular biology or biochemistry laboratory.