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Specifications:
| Application | Protein Electrophoresis & Western Blotting |
| Storage Temperature | -20°C |
| Product Type | DNA Ladders |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ GeneRuler 100 bp DNA Ladder is a versatile molecular weight marker ideal for the sizing and quantification of double-stranded DNA fragments in the range of 100 bp to 1,000 bp. It contains 10 precisely defined DNA fragments, making it ideal for routine agarose gel electrophoresis, including PCR product analysis, genotyping, and cloning verification.
The ladder is supplied with 6X TriTrack DNA Loading Dye, which offers three-color visualization for enhanced tracking during gel runs. The ladder bands are generated from individual, chromatography-purified fragments, ensuring high sharpness, uniform intensity, and quantifiable banding patterns.
Key Features
- DNA size range: 100 bp to 1,000 bp
- 10 individual fragments with defined base pair sizes
- Chromatography-purified DNA for high band clarity and consistency
- Quantifiable bands: defined amounts of DNA per band for approximate DNA concentration estimation
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Includes 6X TriTrack DNA Loading Dye with three indicators:
- Xylene Cyanol FF
- Bromophenol Blue
- Orange G
- Compatible with ethidium bromide or SYBR Safe staining
- Suitable for 1–2% standard or precast agarose gels
DNA Fragment Sizes
100 bp | 200 bp | 300 bp | 400 bp | 500 bp
600 bp | 700 bp | 800 bp | 900 bp | 1,000 bp
Each band has a predefined DNA quantity, allowing for visual quantification of experimental DNA samples when compared side-by-side.
Applications
- Sizing of PCR products
- Estimation of DNA quantity
- Routine DNA analysis on agarose gels
- Fragment verification in cloning workflows
- Electrophoretic tracking in teaching or research labs
Specifications
| Property | Details |
|---|---|
| Product Type | DNA Ladder (double-stranded) |
| Size Range | 100 bp to 1,000 bp |
| Number of Fragments | 10 |
| Concentration | 50 μg per vial |
| Dye Compatibility | Ethidium bromide, SYBR Safe |
| Gel Compatibility | 1–2% agarose or precast agarose gels |
| Loading Dye | 6X TriTrack (includes three dyes) |
| Format | Unstained, not ready to load (add loading dye separately) |
| Ready to Load | No |
| Green Features | Sustainable packaging |
| Unit Size | 50 µg (Cat. No. SM0241), 5 × 50 µg (Cat. No. SM0242) |
Available Formats
| Catalog No. | Quantity |
|---|---|
| SM0241 | 50 μg |
| SM0242 | 5 × 50 μg |
Storage and Handling
- Store at –20°C
- Avoid repeated freeze-thaw cycles
- For research use only; not intended for diagnostic applications
The Thermo Scientific™ GeneRuler 100 bp DNA Ladder is a reliable and high-resolution DNA sizing standard, ideal for routine electrophoresis in any molecular biology laboratory. With its accurately sized fragments, three-color tracking system, and quantifiable banding, it supports both educational and research applications where precision and ease of use are critical.
- Pack Size: 50 µg with 1 mL Loading dye for 100 Applications 5 x 50 µg with 2 x 1 mL Loading dye for 500 Applications