Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific™ DreamTaq DNA Polymerase is a high-performance, enhanced Taq DNA polymerase optimized for a wide range of standard PCR applications. It provides higher sensitivity, longer PCR product amplification, and higher yields compared to conventional Taq DNA polymerases. It is available in both colorless and green formats, making it versatile for routine and specialized applications.

Key Features

1. Enhanced Sensitivity and Yield:
   • Higher sensitivity and yield compared to conventional Taq polymerase.
   • Capable of amplifying long targets:
     • Up to 6 kb from genomic DNA.
     • Up to 20 kb from viral DNA.
2. Streamlined Workflow:
   • Minimal optimization required for robust amplification.
   • Compatible with standard PCR reaction setups and cycling conditions.
3. Two Formats for Flexibility:
   • Colorless Format: For downstream applications such as absorbance or fluorescence excitation analysis.
   • Green Format: Includes a buffer with tracking dyes and density reagents for direct gel loading.
4. Optimized Buffer:
   • Supplied with 10X DreamTaq Buffer, containing 20 mM MgCl2.
   • The green buffer format does not interfere with downstream applications (e.g., sequencing, ligation, digestion).
5. Versatile Applications:
   • Suitable for routine PCR amplification, high-throughput PCR, and genotyping.

Specifications

Applications

1. Routine PCR Amplification:
   • Amplifies DNA fragments up to 6 kb.
2. High-Throughput PCR:
   • Suitable for labs processing large numbers of samples.
3. Genotyping:
   • Ideal for genetic variation detection and analysis.
4. DNA Sequencing Prep:
   • Colorless format allows purification and subsequent sequencing.
5. RNA Analysis Compatibility:
   • DNase- and RNase-free for nucleic acid analysis.

Included Components

1. DreamTaq DNA Polymerase: 40 μL at 5 U/μL.
2. 10X DreamTaq Buffer (20 mM MgCl2): 1.25 mL.

Green vs. Colorless Formats

Comparison of Thermo Scientific™ DreamTaq DNA Polymerase and Promega GoTaq DNA Polymerase:

Summary of Key Differences:

• DreamTaq DNA Polymerase offers extended target length capabilities (up to 7.5 kb for genomic DNA and 20 kb for viral DNA) and enhanced sensitivity.
• GoTaq DNA Polymerase provides flexibility with magnesium-free buffer options (G2 Flexi variant) and is optimized for standard endpoint PCR applications.
• Buffer Options: Both offer green (dye-containing) and colorless options, but DreamTaq’s Green Buffer is explicitly compatible with downstream enzymatic applications.

Storage and Stability

• Storage Temperature: Store at -20°C to maintain enzyme activity.
• Shelf Life: Refer to product packaging for expiration date.

Benefits

1. Improved Performance:
   • Robust amplification across standard PCR applications.
2. Versatility:
   • Ideal for routine and high-sensitivity PCR workflows.
3. Ease of Use:
   • Ready-to-use format with minimal optimization.
4. Efficiency:
   • Amplifies long targets with high yield and reliability.

Ordering Information

Intended Use

• For research use only.
• Not for use in diagnostic procedures.

Thermo Scientific™ DreamTaq DNA Polymerase is an excellent choice for researchers looking for a reliable, high-performance polymerase for PCR applications.