Thermo Fisher Scientific Invitrogen™ Qubit™ 1X Dsdna Hs Assay Kit, 100 Assays Per Kit (copy)

The Invitrogen™ Qubit™ dsDNA Quantification Assay Kits provide a highly selective and reliable method for measuring double-stranded DNA (dsDNA) in a range of sample types. Compatible with Qubit Fluorometers, these kits are ideal for applications requiring accurate quantification of DNA while tolerating contaminants such as salts, solvents, and detergents. They are available in two variants for different sensitivity requirements: High Sensitivity (HS) and Broad Range (BR).

Key Features

1. Accurate Quantification:
   • Designed to selectively measure dsDNA, distinguishing it from single-stranded DNA (ssDNA), RNA, proteins, and free nucleotides.
   • Provides precise readings for low- and high-abundance DNA samples.
2. Wide Dynamic Range:
   • High Sensitivity (HS) Assay Kit:
     • Quantitation range: 0.1–120 ng.
     • Initial DNA concentration range: 0.005–120 ng/μL.
   • Broad Range (BR) Assay Kit:
     • Quantitation range: 4–2000 ng.
     • Initial DNA concentration range: 0.2–2000 ng/μL.
3. Simple Workflow:
   • Mix the concentrated assay reagent with the provided dilution buffer.
   • Add your sample (volume: 1–20 μL).
   • Measure DNA concentration using a Qubit Fluorometer.
4. Compatibility:
   • Designed for use with Qubit Fluorometers, including Qubit 4 and Qubit Flex.
   • Compatible with clear, thin-wall 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex.
5. Robust Contaminant Tolerance:
   • Effectively quantifies dsDNA even in the presence of salts, solvents, and detergents.
6. Flexible Assay Size:
   • Available in packs for 100 or 500 reactions to accommodate different scales of research.

Kit Options

Assay Highlights

High Sensitivity (HS) Assay Kit

• Best for low-abundance samples or sensitive quantification needs.
• Ideal for applications such as sequencing library preparation or small-scale DNA analysis.

Broad Range (BR) Assay Kit

• Best for routine DNA quantification over a broader concentration range.
• Suitable for applications like plasmid prep quantification or genomic DNA analysis.

Specifications

Workflow

1. Prepare the Working Solution:
   • Dilute the concentrated assay reagent with the dilution buffer provided.
2. Add Sample:
   • Mix 1–20 μL of DNA sample with the working solution in the appropriate tube.
3. Measure:
   • Use the Qubit Fluorometer to read the DNA concentration.

Applications

• DNA Sequencing:
  • Quantify DNA prior to library preparation for next-generation sequencing (NGS).
• Genomics Research:
  • Measure genomic DNA for PCR, cloning, or other downstream applications.
• Molecular Biology:
  • Analyze dsDNA content in mixed samples containing RNA, proteins, or free nucleotides.
• Quality Control:
  • Verify the quantity of extracted DNA from various sources.

Notes

• Use only with Qubit Fluorometers for optimal performance.
• Requires appropriate consumables, such as clear, thin-wall PCR tubes or tube strips.
• For research use only; not intended for clinical or diagnostic applications.

Why Choose Qubit dsDNA Assay Kits?

• High specificity for dsDNA ensures accurate quantification without interference.
• Wide quantitation ranges cater to diverse research needs.
• Simplified workflow reduces preparation time, increasing lab efficiency.
• Compatible with a broad range of applications, from basic research to advanced genomics studies.