Human Ceramide Kinase (Cerk) Elisa Kit, 96Tests

The Human Ceramide Kinase (CERK) ELISA Kit is a sensitive and precise tool designed for the quantitative detection of CERK levels in human serum, plasma, culture media, and other biological fluids. Using a Sandwich-ELISA technique, this kit is ideal for research in lipid signaling, ceramide metabolism, and related biological processes.

Purpose

• Quantification of CERK Levels:
  • Measures CERK concentrations as a biomarker for ceramide metabolism and signaling pathways.
• Sample Compatibility:
  • Suitable for human serum, plasma, and various biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the specific and quantitative measurement of CERK. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to CERK.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing CERK in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to CERK is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to reduce background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with CERK.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • CERK concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of CERK with minimal cross-reactivity.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of CERK concentrations using a standard curve.
4. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify the workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• CERK standards for standard curve generation.
• HRP-conjugated CERK detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Lipid Signaling Research:
  • Explore CERK’s role in ceramide and sphingolipid metabolism.
• Metabolic Studies:
  • Investigate CERK as a biomarker in lipid-related metabolic disorders.
• Cellular Signaling Pathways:
  • Study CERK’s involvement in cell signaling and inflammatory responses.
• Drug Development:
  • Assess the impact of therapeutic interventions targeting CERK-related pathways.
• Disease Research:
  • Monitor CERK levels in diseases such as cancer, diabetes, and neurodegenerative conditions.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of CERK.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated CERK antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate CERK concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Ceramide Kinase (CERK) ELISA Kit is a valuable tool for quantifying CERK in biological samples. Its high sensitivity, specificity, and user-friendly design make it an essential resource for lipid metabolism research, cellular signaling studies, and the development of CERK-targeted therapeutics.