Human C-Peptide Elisa Kit, 96 Tests

The Human C-Peptide ELISA Kit is a sensitive and reliable assay designed for the quantitative measurement of C-Peptide levels in human serum, plasma, culture media, and other biological fluids. Utilizing the Sandwich-ELISA technique, this kit provides precise and reproducible results, making it ideal for research in diabetes, pancreatic function, and insulin secretion.

Purpose

• Quantification of C-Peptide Levels:
  • Measures C-Peptide concentration to assess pancreatic beta-cell function and insulin production.
• Sample Compatibility:
  • Designed for use with human serum, plasma, and other biological fluids.

Principle

The assay is based on the Sandwich-ELISA technique, ensuring specificity and accuracy. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to C-Peptide.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing C-Peptide in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to C-Peptide is added, forming a sandwich complex.
4. Washing Step:
   • Unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution is added, reacting with the HRP enzyme to produce a blue color in positive wells.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. The optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Concentration Determination:
   • C-Peptide concentrations are determined by comparing the OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of C-Peptide with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, and other biological fluids.
3. Quantitative Results:
   • Provides accurate measurement of C-Peptide levels using a standard curve.
4. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify assay preparation.
5. Reproducibility:
   • Delivers consistent results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• C-Peptide standards for generating a standard curve.
• HRP-conjugated C-Peptide detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Diabetes Research:
  • Evaluate pancreatic beta-cell function by quantifying C-Peptide levels.
• Endocrine Studies:
  • Monitor insulin secretion and related hormonal activities.
• Metabolic Disorder Research:
  • Investigate metabolic diseases and their effects on C-Peptide levels.
• Drug Development:
  • Assess therapeutic interventions targeting insulin production and secretion.
• Clinical Diagnostics:
  • Support diagnostics for insulinoma and diabetes management.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of C-Peptide.
2. Convenience:
   • Ready-to-use reagents reduce preparation time.
3. Versatility:
   • Compatible with various biological sample types.
4. Reproducibility:
   • Ensures consistent performance across experiments.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated C-Peptide antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate C-Peptide concentration using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles.

The Human C-Peptide ELISA Kit offers a sensitive, accurate, and user-friendly solution for measuring C-Peptide levels in human biological samples. Its specificity and versatility make it an essential tool for diabetes research, metabolic studies, and clinical diagnostics.