Thermo Scientific™ Remel™ BactiDrop™ Oxidase, 0.75

Thermo Scientific™ Remel™ BactiDrop™ Oxidase

Thermo Scientific™ Remel™ BactiDrop™ Oxidase is a ready-to-use diagnostic reagent designed to detect the presence of the cytochrome oxidase enzyme in bacterial isolates. This test is used in clinical and research laboratories to differentiate between oxidase-positive and oxidase-negative microorganisms, supporting bacterial identification processes.

Key Features

1. Cytochrome Oxidase Detection: Specifically identifies the bacterial production of the oxidase enzyme, essential for distinguishing oxidase-positive microorganisms.
2. Ready-to-Use: No preparation required, simplifying workflow in busy laboratory environments.
3. Easy Interpretation:
   • Positive Test: Development of violet to purple color within 10–30 seconds.
   • Negative Test: Delayed reaction or no color change within 10–30 seconds.
4. Stable and Reliable: Contains ascorbic acid to reduce auto-oxidation, ensuring stability and accuracy.
5. Precise Formulation: Based on the oxidase reagent formula of Kovacs, ensuring consistent and reproducible results.

Applications

• Clinical Diagnostics: Used to identify Neisseria species and differentiate Pseudomonadaceae from oxidase-negative Enterobacteriaceae.
• Microbial Research: Distinguishes between oxidase-positive Gram-negative bacteria and oxidase-negative Gram-positive bacteria.
• Food and Water Testing: Identifies oxidase-positive bacteria in environmental and food samples.
• Veterinary Microbiology: Supports bacterial differentiation in animal health diagnostics.

How It Works

The oxidase test detects the presence of a cytochrome oxidase system, which activates the oxidation of reduced cytochrome c by molecular oxygen. This reaction produces a purple-colored compound, indophenol, in oxidase-positive bacteria.

Reaction Components:

• N,N,N,N-tetramethyl-1,4-phenylenediamine: Serves as the primary reagent for oxidase activity.
• Ascorbic Acid: Enhances stability by reducing auto-oxidation.
• Demineralized Water: Ensures reagent consistency.

Product Details

• Formulation: 0.75 mL ampules of oxidase reagent.
• Components:
  • N,N,N,N-tetramethyl-1,4-phenylenediamine (CAS 100-22-1)
  • Ascorbic Acid (CAS 50-81-7)
  • Demineralized Water (CAS 7732-18-5)
• Packaging: Supplied in convenient ampules for easy and precise application.

Test Procedure

1. Apply Reagent: Dispense a drop of reagent onto filter paper or directly onto bacterial colonies on an appropriate medium.
2. Observe Reaction:
   • Positive: Violet to purple color within 10–30 seconds.
   • Negative: No color change or delayed reaction.
3. Interpret Results: Record findings based on the color change timeframe.

Safety and Handling

• For in vitro diagnostic use only.
• Handle with care, using appropriate personal protective equipment (PPE).
• Dispose of ampules and reagents in accordance with local biohazard waste regulations.

Storage and Stability

• Store at 2–8°C to maintain reagent stability.
• Refer to product packaging for expiration date.

Applications in Microbial Differentiation

• Differentiates between oxidase-positive Pseudomonas spp. and oxidase-negative Enterobacteriaceae.
• Identifies oxidase-positive Neisseria spp..
• Used in environmental microbiology to detect oxidase activity in Gram-negative bacteria.

Thermo Scientific™ Remel™ BactiDrop™ Oxidase is a trusted tool for clinical, food, water, and veterinary microbiology, enabling precise and efficient detection of oxidase-positive bacteria.