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Thermo Scientific™ Oxoid™ Membrane Endo Agar LES (Dehydrated)
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for the enumeration of coliform organisms in water using a 2-stage enrichment technique.
Specifications:
| Application | Microbiology, Water Quality Analysis | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | Target Organism Class | coliform organisms |
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Membrane Endo Agar LES (Dehydrated) is a selective and differential medium designed for the enumeration of coliform organisms in water samples using the membrane filtration technique. Prepared according to the Lawrence Experimental Station (LES) formulation, this medium provides enhanced recovery and resuscitation of coliforms, offering greater precision and reliability compared to traditional MPN (Most Probable Number) methods.
Key Features
- Highly Selective Medium:
- Differentiates coliforms based on their ability to produce colonies with a golden-green metallic sheen.
- Optimized for Water Testing:
- Designed for use in testing potable water, non-potable water, and sewage effluents.
- Membrane Filtration:
- Suitable for membrane filtration methods, ensuring precise and reliable enumeration.
- Supports Resuscitation:
- Incorporates a two-stage enrichment process to resuscitate stressed coliforms, enhancing recovery.
- APHA-Compliant:
- Meets the standards outlined by the American Public Health Association (APHA) for water quality testing.
Applications
1. Water Microbiology
- Enumeration of coliforms in drinking water, recreational water, and wastewater.
2. Environmental Monitoring
- Detection of coliform contamination in water systems, industrial effluents, and public water supplies.
3. Public Health and Compliance Testing
- Ensures compliance with water safety and quality standards by identifying potential coliform contamination.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Membrane Endo Agar LES |
| Applications | Water microbiology and environmental testing |
| Quantity | 500g |
| Yield | 11.1L of prepared medium |
| Compliance | APHA standards |
Preparation Instructions
- Suspend: Suspend 50g of Membrane Endo Agar LES powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved. Do not autoclave.
- Cool and Add Fuchsin: After cooling to 45–50°C, aseptically add Basic Fuchsin (BR0050) as specified.
- Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- High Recovery Rates: Enhanced recovery of stressed coliforms using a two-stage enrichment technique.
- Clear Differentiation: Coliforms form colonies with a golden-green metallic sheen for easy identification.
- Precise Enumeration: Membrane filtration provides greater reliability and precision compared to MPN methods.
- Regulatory Compliance: Meets APHA standards for water microbiology workflows.
- Cost-Effective: High yield of 11.1L per 500g, suitable for routine water testing.
Diagnostic Features
| Organism | Reaction |
|---|---|
| Coliforms | Golden-green metallic sheen on colonies |
| Non-Coliforms | Varying colony appearance or no sheen |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Membrane Endo Agar LES (Dehydrated)
- Catalog Number: MM0551B
- Quantity: 500g
- Yield: 11.1L of prepared medium
The Thermo Scientific™ Oxoid™ Membrane Endo Agar LES (Dehydrated) is a trusted and effective medium for the enumeration of coliforms in water samples. Its precision, reliability, and compliance with APHA standards make it an essential tool for laboratories focused on water safety, environmental monitoring, and public health.