Invitrogen™ Cytokine 10-Plex Human Panel

The Invitrogen™ Cytokine 10-Plex Human Panel for the Luminex™ platform provides a comprehensive, efficient, and highly sensitive tool for the simultaneous quantification of 10 human cytokines in serum, plasma, and cell culture supernatants. Using magnetic bead-based multiplexing technology, this panel reduces hands-on time and increases throughput compared to traditional methods like ELISA.

Key Features:

1. Multiplex Detection:
   • Measures 10 analytes in a single assay:
     • GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF-α.
2. Luminex™ xMAP™ Technology:
   • Enables differentiation and quantification of multiple cytokines in the same well using bead-based suspension technology.
3. Magnetic Beads:
   • Simplify assay wash steps, enable automation, and ensure uniform results for higher reproducibility.
4. Fast and Flexible Protocol:
   • Obtain results in as little as 3.5 hours.
5. Compatibility:
   • Suitable for use with Luminex™ MAGPIX™, 200™, FLEXMAP 3D™, and other xMAP™ platforms.

Specifications:

Applications:

1. Immunology Research:
   • Measure key cytokines involved in immune response regulation.
2. Inflammatory Disease Studies:
   • Profile cytokines in conditions such as autoimmune diseases, sepsis, and inflammatory disorders.
3. Cancer Research:
   • Investigate cytokine-mediated tumor microenvironments and immune responses.
4. Biomarker Discovery:
   • Identify and quantify cytokine signatures in clinical or preclinical research.
5. Drug Development:
   • Evaluate the efficacy and safety of immunomodulatory therapies by monitoring cytokine levels.

Advantages:

1. Comprehensive Analysis:
   • Quantify 10 cytokines simultaneously, saving time and resources compared to individual ELISAs.
2. High Sensitivity and Specificity:
   • Uses fully qualified antibodies for accurate and reliable results.
3. Automation-Ready:
   • Magnetic bead-based design simplifies wash steps, enabling automated workflows.
4. Validated Performance:
   • Rigorous validation ensures reproducibility and confidence in results.
5. Cost-Effective:
   • Reduces reagent use and sample volume while maximizing data output.

Protocol Overview:

1. Prepare Samples and Standards:
   • Dilute samples and reconstitute standards according to the provided protocol.
2. Add Samples to Plate:
   • Add premixed antibody-coated capture beads to wells and incubate with samples.
3. Wash and Add Detection Antibody:
   • Wash beads and add the premixed detection antibody.
4. Add Streptavidin-R-PE:
   • Incubate with SAV-R-PE to complete the sandwich immunoassay.
5. Wash and Analyze:
   • Wash beads, resuspend, and analyze using a compatible Luminex™ instrument.

Contents:

• Premixed antibody-coated capture beads.
• Premixed detector antibody.
• Standards and SAV concentrate.
• Buffers, wash solution, and diluents.
• 96-well flat-bottom plate.
• Complete protocol and lot-specific technical data sheet.

Ordering Information:

Storage and Handling:

• Store at 2°C to 8°C.
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

Related Products:

• Luminex™ MAGPIX™ System: Compatible instrument for multiplex assays.
• Cytokine 30-Plex Human Panel: For expanded cytokine profiling.
• Customizable Luminex™ Panels: Build a panel tailored to specific research needs.

The Invitrogen™ Cytokine 10-Plex Human Panel provides a comprehensive, high-performance solution for profiling key cytokines in human samples. Its compatibility with Luminex™ platforms, ease of use, and robust performance make it an essential tool for immunology, inflammation, and cancer research.