Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X) is a ready-to-use reducing solution designed for use in protein sample preparation for SDS-PAGE. It contains 500 mM dithiothreitol (DTT) in a stabilized liquid form, eliminating the need to weigh and dissolve dry DTT powder.

Used at a 1:10 dilution, this reagent ensures effective reduction of disulfide bonds in protein samples, aiding in complete protein denaturation and optimal migration during electrophoresis on NuPAGE™ Bis-Tris gels and other denaturing gel systems. Its compatibility with NuPAGE™ LDS Sample Buffer and electrophoresis workflows makes it a staple reagent for Western blotting and protein analysis.

Key Features and Benefits

• Convenient liquid format – no weighing or dissolution required
• High concentration (500 mM) – provides effective reduction at 1X working concentration
• Improves protein unfolding – ensures accurate migration during SDS-PAGE
• Stabilized DTT formulation – extended shelf life with reduced degradation
• Optimized for use with NuPAGE™ LDS Sample Buffer and Bis-Tris gels

Applications

• Protein reduction for SDS-PAGE and Western blotting
• Preparation of samples for mass spectrometry and sequencing
• Denaturation of complex proteins for analysis
• Compatible with NuPAGE™, Bolt™, and Tris-Glycine gel systems

Technical Specifications

NuPAGE™ Sample Reducing Agent (10X) offers a fast, effective, and reliable solution for reducing protein disulfide bonds prior to electrophoresis. With a convenient liquid format and stabilized formulation, it delivers consistent performance for all protein analysis workflows requiring accurate molecular weight determination and Western blotting.