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Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC
Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody is designed for use in flow cytometry applications. The antibody detects CD45RA, a marker for subsets of human leukocytes, using a high-quality FITC (fluorescein isothiocyanate) conjugate for fluorescence detection.
Key Features
- Target Information:
- Reacts with CD45RA, a variant of the CD45 antigen expressed on subsets of human leukocytes.
- Marker for naive T cells and subsets of B cells.
- Applications:
- Optimized for flow cytometry.
- Pre-titrated and tested for use on human peripheral blood cells.
- Fluorescence Detection:
- Conjugated to FITC with Excitation/Emission Max of 488/519 nm.
- Designed for use with blue laser (488 nm) in flow cytometry.
- Host and Isotype:
- Produced in mouse, IgG2b kappa.
- Purity and Formulation:
- Purified via affinity chromatography.
- Provided as a liquid formulation in PBS (pH 7.2) with 0.2% BSA and 0.09% sodium azide.
Specifications
| Property | Details |
|---|---|
| Clone | HI100 |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Concentration | 5 µL/test (0.015 µg/test) |
| Formulation | PBS with 0.2% BSA and 0.09% sodium azide |
| Applications | Flow cytometry |
| Excitation/Emission | 488/519 nm |
| Storage Conditions | 4°C, protected from light, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| RRID | AB_1582285 |
| Regulatory Status | For Research Use Only; not for diagnostic procedures |
Applications
- Flow Cytometry:
- Detection and analysis of human CD45RA+ leukocytes.
- Staining of naive T cells and subsets of B cells in human peripheral blood.
- Immunophenotyping:
- Identification of immune cell populations in research studies.
- Immune Response Research:
- Used to study T cell maturation and immune function.
Preparation and Handling
- Storage: Store at 4°C in the dark. Avoid freezing as it may compromise performance.
- Light Sensitivity: Protect from light to maintain dye fluorescence integrity.
- Fixation Compatibility: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days.
Additional Notes
- Light Source Compatibility: Designed for excitation with blue laser (488 nm) and emission detection at 519 nm.
- Sample Fixation: Recommended fixation buffers include Invitrogen IC Fixation Buffer (Product # 00-822-49).
- Panel Builder: Use Invitrogen's Flow Cytometry Panel Builder for designing customized multicolor panels.
The Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC is a reliable, high-quality reagent for identifying and characterizing CD45RA+ cells in flow cytometry. Its FITC conjugation ensures consistent fluorescence performance, making it an essential tool for immune cell analysis in research studies.
No resources are currently available for this product.
Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC
Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody is designed for use in flow cytometry applications. The antibody detects CD45RA, a marker for subsets of human leukocytes, using a high-quality FITC (fluorescein isothiocyanate) conjugate for fluorescence detection.
Key Features
- Target Information:
- Reacts with CD45RA, a variant of the CD45 antigen expressed on subsets of human leukocytes.
- Marker for naive T cells and subsets of B cells.
- Applications:
- Optimized for flow cytometry.
- Pre-titrated and tested for use on human peripheral blood cells.
- Fluorescence Detection:
- Conjugated to FITC with Excitation/Emission Max of 488/519 nm.
- Designed for use with blue laser (488 nm) in flow cytometry.
- Host and Isotype:
- Produced in mouse, IgG2b kappa.
- Purity and Formulation:
- Purified via affinity chromatography.
- Provided as a liquid formulation in PBS (pH 7.2) with 0.2% BSA and 0.09% sodium azide.
Specifications
| Property | Details |
|---|---|
| Clone | HI100 |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Concentration | 5 µL/test (0.015 µg/test) |
| Formulation | PBS with 0.2% BSA and 0.09% sodium azide |
| Applications | Flow cytometry |
| Excitation/Emission | 488/519 nm |
| Storage Conditions | 4°C, protected from light, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| RRID | AB_1582285 |
| Regulatory Status | For Research Use Only; not for diagnostic procedures |
Applications
- Flow Cytometry:
- Detection and analysis of human CD45RA+ leukocytes.
- Staining of naive T cells and subsets of B cells in human peripheral blood.
- Immunophenotyping:
- Identification of immune cell populations in research studies.
- Immune Response Research:
- Used to study T cell maturation and immune function.
Preparation and Handling
- Storage: Store at 4°C in the dark. Avoid freezing as it may compromise performance.
- Light Sensitivity: Protect from light to maintain dye fluorescence integrity.
- Fixation Compatibility: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days.
Additional Notes
- Light Source Compatibility: Designed for excitation with blue laser (488 nm) and emission detection at 519 nm.
- Sample Fixation: Recommended fixation buffers include Invitrogen IC Fixation Buffer (Product # 00-822-49).
- Panel Builder: Use Invitrogen's Flow Cytometry Panel Builder for designing customized multicolor panels.
The Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC is a reliable, high-quality reagent for identifying and characterizing CD45RA+ cells in flow cytometry. Its FITC conjugation ensures consistent fluorescence performance, making it an essential tool for immune cell analysis in research studies.
No resources are currently available for this product.
Spezifikationen
| Pack Size | For 100 Tests, for 25 Tests |