Invitrogen™ Cal-Lyse™ Lysing Solution, Whole Blood Lysing Solution

Optimized Erythrocyte Lysis for Human Peripheral Blood in Flow Cytometry Applications

Invitrogen™ Cal-Lyse™ is a fast and effective whole blood lysing solution specifically formulated for the selective removal of red blood cells (RBCs) from anticoagulated human peripheral blood samples. It enables high-quality leukocyte enumeration in samples prepared for flow cytometric analysis using fluorochrome-conjugated monoclonal antibodies.

This reagent supports rapid lysis with minimal leukocyte loss, delivering clean, reproducible results for immunophenotyping and hematological studies.

Key Features and Benefits

• Selective Erythrocyte Lysis
  Effectively removes red blood cells from whole blood without compromising leukocyte integrity.
• Flow Cytometry-Compatible
  Ideal for preparing antibody-stained whole blood samples for multi-parameter flow cytometry.
• Simple and Ready to Use
  Provided in a convenient liquid format, requiring no dilution or additional reagents.
• Preserves Cell Surface Markers
  Maintains antigen expression for accurate detection and quantification of leukocyte subsets.

Applications

• Whole blood sample preparation for flow cytometry
• Leukocyte immunophenotyping
• CD marker analysis in human peripheral blood
• Clinical research involving immune cell subsets

Intended for use with anticoagulated human blood samples only.

Technical Specifications

Available Pack Sizes

Invitrogen™ Cal-Lyse™ Whole Blood Lysing Solution offers a reliable and user-friendly method for RBC lysis in human peripheral blood samples. It is ideally suited for preparing stained whole blood for flow cytometric analysis, providing excellent cell preservation and reproducibility for immunology and hematology research.