Gibco™ Opti-MEM™ I Reduced Serum Medium

The Gibco™ Opti-MEM™ I Reduced Serum Medium is an optimized Minimal Essential Medium (MEM) that enables at least a 50% reduction in fetal bovine serum (FBS) supplementation while maintaining normal cell growth and morphology. This medium is widely used in transfection applications and supports the culture of various suspension and adherent mammalian cell lines, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.

Opti-MEM™ I contains additional nutrients, such as insulin, transferrin, hypoxanthine, thymidine, and trace elements, making it a superior alternative to standard MEM for reduced-serum conditions.

Key Features & Benefits

1. Reduced Serum Requirements

• Allows for at least a 50% reduction in FBS supplementation, lowering culture costs.
• Maintains normal cell growth and morphology, even with reduced serum levels.

2. Optimized for Transfection & Mammalian Cell Culture

• Recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent.
• Supports the growth of a variety of cell lines, including CHO, BHK-21, HEK, Sp2, and AE-1.
• Contains L-glutamine for enhanced cell viability and phenol red as a pH indicator.

3. Enhanced Nutrient Composition for Improved Performance

• Contains insulin, transferrin, hypoxanthine, thymidine, and trace elements, reducing the need for additional supplements.
• Uses a sodium bicarbonate buffer system (2.4 g/L), requiring a 5–10% CO₂ environment to maintain physiological pH.

4. High-Quality, Sterile-Filtered & cGMP Compliant

• Sterile-filtered for reliable cell culture conditions.
• Manufactured under cGMP-compliant and ISO 13485-certified processes.
• Shelf life: 18 months from date of manufacture.

Specifications

Available Product Variants

Applications

1. Cell Culture & Reduced Serum Growth

• Supports a variety of mammalian cell lines, including CHO, BHK-21, and HEK.
• Optimized for adherent and suspension cultures under reduced-serum conditions.

2. Transfection & Molecular Biology

• Recommended for use with Lipofectamine™ transfection reagents.
• Ideal for DNA, RNA, and protein expression studies.

3. Virus Production & Cytotoxicity Assays

• Enhances virus growth and propagation under controlled conditions.
• Used for sample testing in cytotoxicity and cell viability assays.

Storage & Handling

• Store at 2–8°C and protect from light to maintain stability.
• Do not freeze to prevent degradation of components.
• Ensure proper CO₂ regulation (5–10%) to maintain pH balance in cell culture.

Ordering Information

The Gibco™ Opti-MEM™ I Reduced Serum Medium is a high-performance alternative to standard MEM, allowing for at least a 50% reduction in fetal bovine serum (FBS) supplementation while maintaining optimal cell growth and viability. With added nutrients and optimized composition, this medium is ideal for cell culture, transfection, virus production, and molecular biology applications.