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Gibco™ Hybridoma-SFM (Serum-Free Medium) is a complete, ready-to-use, low-protein, serum-free culture medium formulated to support the growth and productivity of hybridomas and recombinant myeloma cell lines for monoclonal antibody (mAb) production. With less than 20 µg/mL total protein, Hybridoma-SFM minimizes background protein content, greatly simplifying downstream antibody purification and enhancing reproducibility in manufacturing-scale workflows.
This medium supports both suspension and stationary cultures, including high-performance hybridomas and cholesterol-dependent lines such as NS0 (with the addition of 250X Cholesterol Lipid Concentrate). It contains L-glutamine and phenol red, offering immediate usability without the need for further supplementation.
Manufactured under cGMP-compliant conditions at a FDA-registered, ISO 13485-certified facility, Gibco™ Hybridoma-SFM is suitable for regulated research and commercial production environments. A Type II Drug Master File (DMF) is available for FDA-regulated customers upon request.
Key Features
- Serum-Free & Low-Protein: Contains <20 µg/mL total protein—ideal for mAb purification.
- Complete & Ready to Use: Pre-supplemented with glutamine and phenol red for convenient use.
- Supports Hybridomas and Myelomas: Optimized for both traditional and recombinant lines.
- High IgG Productivity: Promotes robust antibody yields in scalable formats.
- Regulatory Friendly: Manufactured in a cGMP-compliant, ISO-certified facility with DMF support.
Applications
-
Monoclonal Antibody Production:
- Expansion of hybridomas and recombinant myeloma cells.
-
Suspension and Adherent Cultures:
- Supports stationary and suspension cell lines.
-
Serum-Free Bioprocessing:
- Facilitates purification workflows and reduces downstream burden.
-
GMP-Compatible Cell Culture:
- For preclinical and further manufacturing use.
Specifications
| Attribute | Description |
|---|---|
| Cell Type | Hybridomas, Myelomas |
| Form | Liquid |
| Serum Level | Serum-Free |
| Protein Content | <20 µg/mL |
| Additives | With Glutamine, With Phenol Red |
| Supplementation Needed | None (ready-to-use) |
| Classification | Low-Protein, Chemically Defined, Serum-Free |
| Product Line | Gibco™ |
| Storage Conditions | 2–8°C, protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months |
| Regulatory Support | FDA Type II DMF available upon request |
| Manufacturing Facility | Grand Island, NY – cGMP-compliant, ISO 13485 |
| Intended Use | Research Use or Further Manufacturing Only |
Gibco™ Hybridoma-SFM provides a clean, serum-free solution for hybridoma culture, optimizing monoclonal antibody production while simplifying purification and reducing variability. Its regulatory readiness and high-performance profile make it ideal for antibody discovery, preclinical development, and commercial-scale workflows.
Brochure
Hybridoma Insert
hybridoma SFM
Gibco™ Hybridoma-SFM (Serum-Free Medium) is a complete, ready-to-use, low-protein, serum-free culture medium formulated to support the growth and productivity of hybridomas and recombinant myeloma cell lines for monoclonal antibody (mAb) production. With less than 20 µg/mL total protein, Hybridoma-SFM minimizes background protein content, greatly simplifying downstream antibody purification and enhancing reproducibility in manufacturing-scale workflows.
This medium supports both suspension and stationary cultures, including high-performance hybridomas and cholesterol-dependent lines such as NS0 (with the addition of 250X Cholesterol Lipid Concentrate). It contains L-glutamine and phenol red, offering immediate usability without the need for further supplementation.
Manufactured under cGMP-compliant conditions at a FDA-registered, ISO 13485-certified facility, Gibco™ Hybridoma-SFM is suitable for regulated research and commercial production environments. A Type II Drug Master File (DMF) is available for FDA-regulated customers upon request.
Key Features
- Serum-Free & Low-Protein: Contains <20 µg/mL total protein—ideal for mAb purification.
- Complete & Ready to Use: Pre-supplemented with glutamine and phenol red for convenient use.
- Supports Hybridomas and Myelomas: Optimized for both traditional and recombinant lines.
- High IgG Productivity: Promotes robust antibody yields in scalable formats.
- Regulatory Friendly: Manufactured in a cGMP-compliant, ISO-certified facility with DMF support.
Applications
-
Monoclonal Antibody Production:
- Expansion of hybridomas and recombinant myeloma cells.
-
Suspension and Adherent Cultures:
- Supports stationary and suspension cell lines.
-
Serum-Free Bioprocessing:
- Facilitates purification workflows and reduces downstream burden.
-
GMP-Compatible Cell Culture:
- For preclinical and further manufacturing use.
Specifications
| Attribute | Description |
|---|---|
| Cell Type | Hybridomas, Myelomas |
| Form | Liquid |
| Serum Level | Serum-Free |
| Protein Content | <20 µg/mL |
| Additives | With Glutamine, With Phenol Red |
| Supplementation Needed | None (ready-to-use) |
| Classification | Low-Protein, Chemically Defined, Serum-Free |
| Product Line | Gibco™ |
| Storage Conditions | 2–8°C, protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months |
| Regulatory Support | FDA Type II DMF available upon request |
| Manufacturing Facility | Grand Island, NY – cGMP-compliant, ISO 13485 |
| Intended Use | Research Use or Further Manufacturing Only |
Gibco™ Hybridoma-SFM provides a clean, serum-free solution for hybridoma culture, optimizing monoclonal antibody production while simplifying purification and reducing variability. Its regulatory readiness and high-performance profile make it ideal for antibody discovery, preclinical development, and commercial-scale workflows.
Brochure
Hybridoma Insert
hybridoma SFM
Spezifikationen
| Pack Size | 1L, 500 mL |