Gibco™ Zeocin™ Selection Reagent

High-Potency Glycopeptide Antibiotic for Stable Selection in Mammalian, Insect, Yeast, Bacterial, and Plant Cells

Gibco™ Zeocin™ Selection Reagent is a sterile-filtered liquid formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin™ belongs to the bleomycin/phleomycin family and exerts its cytotoxic activity by binding to and cleaving DNA, which leads to rapid cell death in non-resistant cells. This mechanism makes it highly effective for selecting genetically modified cells that express a Zeocin resistance gene (Sh ble), commonly included in many expression and transfection vectors.

Supplied as a 100 mg/mL solution in sterile water, Zeocin™ is suitable for use in a wide range of cell types and organisms, including mammalian cells, insect cells, yeast, bacteria, and plant cultures.

Key Features and Benefits

• High-Activity Glycopeptide Antibiotic
  Contains phleomycin D1, which causes double-stranded DNA breaks, leading to rapid cytotoxicity in sensitive cells.
• Broad Compatibility
  Effective for selection in mammalian, insect, yeast, bacterial, and plant systems—ideal for multi-platform workflows.
• Rapid and Reliable Selection
  Enables efficient elimination of non-transfected or non-transformed cells, ensuring only stably modified cells survive.
• Convenient Ready-to-Use Format
  Supplied as a 100 mg/mL sterile-filtered solution, eliminating the need for reconstitution or filtration.
• Stable and Easy to Store
  Ships on ice and should be stored at –5°C to –20°C, with a long shelf life under proper conditions.

Applications

• Selection of mammalian and insect cells carrying the Sh ble resistance gene
• Generation of stable cell lines following plasmid transfection
• Antibiotic selection in yeast and E. coli transformation workflows
• Transgenic plant selection using Zeocin-resistant vectors
• Dual-antibiotic selection with other agents like Hygromycin B or Blasticidin

Recommended Working Concentrations

A kill curve is recommended to determine the optimal concentration for your specific cell type.

Technical Specifications

Gibco™ Zeocin™ Selection Reagent is a powerful and versatile antibiotic that offers researchers a dependable method for eliminating non-transfected cells across a wide variety of host organisms. Whether you're generating mammalian stable lines, performing yeast transformations, or engineering transgenic plants, Zeocin™ provides consistent and potent selection pressure backed by Thermo Fisher’s renowned quality standards.