Gibco™ RPMI(Roswell Park Memorial Institute ) 1640 Medium, X1, 500mL

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

• With
  • Glutamine
  • Phenol Red
• Without
  • HEPES

The complete formulation is available.

Using RPMI

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

Specifications

• Culture Environment: CO2
• Cell Type: Mammalian Cells
• Classification: Animal Origin-free
• Concentration: 1 X
• Culture Type: Mammalian Cell Culture
• Form: Liquid
• Product Type: RPMI 1640
• Serum Level: Standard Serum Supplementation
• Shelf Life: 12 Months
• Sterility: Sterile-filtered
• With Additives: Glutamine, Phenol Red, Sodium Bicarbonate
• Without Additives: No HEPES, No Sodium Pyruvate
• Osmolality: 260 - 310 mOsm/kg
• pH: 7 to 7.4
• Product Line: Gibco™
• Storage conditions: 2-8° C. Protect from light.
• Shipping conditions: Ambient.
• Shelf life: 12 months from date of manufacture.