Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

The Gibco™ RPMI 1640 Medium with GlutaMAX™ Supplement and HEPES is a modified version of the classic RPMI 1640, initially developed to culture human leukemic cells. It is now widely used for the culture of a variety of mammalian cell types such as HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, and carcinomas. This medium has been modified to provide enhanced cell growth and viability, making it suitable for a wide range of cell culture applications.

Key Features:

1. GlutaMAX™ Supplement:
   • GlutaMAX™ I reduces the toxic build-up of ammonia in the culture media, improving cell viability and growth, especially in cultures where glutamine supplementation is needed.
2. HEPES Buffer:
   • The addition of HEPES helps maintain a stable pH under various culture conditions, making it ideal for mammalian cells that require stable pH for optimal growth.
3. High Vitamin Concentration:
   • RPMI 1640 contains a high concentration of vitamins, including biotin, vitamin B12, and PABA, as well as inositol and choline, making it superior in supporting cellular metabolism.
4. Customizable Supplementation:
   • Serum supplementation (typically 10% Fetal Bovine Serum (FBS)) is required for optimal cell growth. The lack of proteins, lipids, and growth factors allows users to customize supplementation for specific needs.
5. No Proteins or Growth Factors:
   • RPMI 1640 does not contain proteins, lipids, or growth factors, allowing for more control over the culture conditions and minimizing the risk of contamination from undefined substances.
6. Sterile-Filtered:
   • The medium is sterile-filtered, ensuring a contamination-free environment for sensitive cell cultures.
7. Shipping and Storage:
   • Ambient shipping conditions and storage at 2-8°C help maintain the integrity of the medium, ensuring optimal performance.
8. Long Shelf Life:
   • The medium comes with a 12-month shelf life from the date of manufacture, ensuring long-term stability.

Specifications:

Applications:

1. Cell Culture:
   • Ideal for growing a wide range of mammalian cells including leukemic cell lines, primary cell cultures, and immortalized cell lines.
2. Cancer Research:
   • Used in cancer research to grow carcinoma cells like MCF-7 for drug testing and cell cycle studies.
3. Immunology and Virology:
   • Suitable for PBMC culture, immunology studies, and viral infection experiments.
4. Metabolic Studies:
   • Often used in metabolic studies and protein production due to its high vitamin content and stable pH.
5. Regenerative Medicine:
   • Used for stem cell culture and regenerative medicine applications where controlled and reproducible culture conditions are required.
6. Toxicology:
   • Applied in toxicology research for testing the impact of chemicals on cellular function.

Catalog Numbers:

• 72400112: 20 x 100 mL, GlutaMAX™, HEPES, Phenol Red, No Sodium Pyruvate
• 72400013: 100 mL, GlutaMAX™, HEPES, Phenol Red, No Sodium Pyruvate
• 72400021: 500 mL, GlutaMAX™, HEPES, Phenol Red, Sodium Bicarbonate, No Sodium Pyruvate
• 72400054: 10 x 500 mL, GlutaMAX™, HEPES, Phenol Red, Sodium Bicarbonate, No Sodium Pyruvate

The Gibco™ RPMI 1640 Medium with GlutaMAX™ Supplement and HEPES is an advanced, highly reliable medium used for a wide range of cell culture applications. With its optimized composition, it provides the necessary nutrients for cell growth, metabolic studies, and research applications in pharmaceuticals, biotechnology, immunology, and environmental research. The addition of GlutaMAX™ ensures reduced ammonia toxicity, while HEPES ensures pH stability, making it the ideal choice for labs that need consistent and reproducible results.