Gibco™ Human PDGF-BB Recombinant Protein, PeproTech®

Gibco™ Human PDGF-BB Recombinant Protein (PeproTech®) is a highly purified recombinant growth factor widely used in cell biology, regenerative medicine, and biomedical research to study cellular proliferation, migration, and signaling pathways. PDGF-BB is one of the most potent isoforms of the platelet-derived growth factor family and plays a critical role in regulating the growth and survival of mesenchymal cells, including fibroblasts, smooth muscle cells, and glial cells.

This recombinant protein is produced using an E. coli expression system and supplied as a lyophilized powder to ensure long-term stability and convenient storage. Recombinant Human PDGF-BB forms a disulfide-linked homodimer composed of two beta chains, with a total molecular weight of approximately 24.3 kDa. The protein is purified to ≥98% purity by SDS-PAGE and HPLC analysis, ensuring high reliability for sensitive research applications.

PDGF-BB exhibits strong biological activity by stimulating the dose-dependent proliferation of Balb/c 3T3 fibroblast cells, with an expected ED50 of 1.0–3.0 ng/mL. Due to its high potency and low endotoxin level (<1 EU/µg), this protein is suitable for a wide range of cell culture and signaling studies.

Key Features

• Recombinant human PDGF-BB growth factor
• High purity (≥98%) confirmed by SDS-PAGE and HPLC
• Produced in E. coli expression system
• Disulfide-linked homodimer consisting of two B chains
• Highly potent biological activity (ED50: 1.0–3.0 ng/mL)
• Low endotoxin level (<1 EU/µg) suitable for cell culture experiments
• Supplied as a lyophilized, preservative-free protein
• Widely cited in scientific literature and validated in numerous research studies

Applications

• Cell proliferation and growth factor signaling studies
• Fibroblast and smooth muscle cell culture
• Stem cell and regenerative medicine research
• Cancer biology and tumor microenvironment studies
• Tissue repair and wound healing research
• Cytokine stimulation assays
• ELISA standards and Western blot controls
• Angiogenesis and vascular biology research

Available Sizes

Technical Specifications

Biological Function

Platelet-derived growth factor (PDGF) is a key regulator of cell growth, chemotaxis, and tissue remodeling. PDGF-BB is a homodimeric growth factor composed of two PDGF-B chains and is capable of activating both PDGF receptor-α and PDGF receptor-β signaling pathways.

PDGF-BB is involved in several important biological processes, including:

• Stimulation of fibroblast and smooth muscle cell proliferation
• Regulation of extracellular matrix synthesis and collagen production
• Promotion of angiogenesis and vascular remodeling
• Regulation of immune cell migration and inflammatory responses
• Support of neuronal survival and glial cell proliferation

These activities make PDGF-BB a critical reagent in cell signaling research, regenerative medicine, oncology, and vascular biology.

Gibco™ Human PDGF-BB Recombinant Protein (PeproTech®) provides researchers with a reliable and highly bioactive cytokine for investigating PDGF-mediated signaling pathways and cellular responses. With exceptional purity, strong biological activity, and low endotoxin levels, this recombinant protein is an essential tool for advanced cell culture experiments, growth factor research, and regenerative medicine studies.