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Thermo Scientific™ DNase I, RNase-Free is a high-purity endonuclease that digests single- and double-stranded DNA, breaking down phosphodiester bonds to produce mono- and oligodeoxyribonucleotides with 5′-phosphate and 3′-OH groups. The enzyme is recombinant and purified from a non-animal host, ensuring minimal intrinsic RNase contamination, making it ideal for RNA preparation and molecular biology applications.
The enzyme’s activity is strictly dependent on Ca²⁺ ions and is activated by Mg²⁺ or Mn²⁺ ions, allowing for different cleavage patterns depending on the cation present:
- Mg²⁺: Cleaves each DNA strand independently, generating randomly fragmented DNA.
- Mn²⁺: Cleaves both DNA strands at the same site, producing blunt-ended or slightly overhanging DNA fragments.
Key Features & Benefits
1. High-Purity Recombinant Enzyme
- RNase-free, ensuring DNA digestion without affecting RNA integrity.
- Produced in a non-animal host, reducing contamination risks.
- High specificity and efficiency for DNA degradation.
2. Versatile DNA Digestion Capabilities
- Cleaves single- and double-stranded DNA into short fragments.
- Generates blunt or overhanging DNA ends, depending on divalent cation conditions.
- Works in a variety of reaction buffers, enhancing compatibility with different workflows.
3. Optimized for RNA Preparation & Molecular Biology
- Removes DNA contamination from RNA samples before RT-PCR or RT-qPCR.
- Suitable for DNA footprinting, nick-translation, and DNA library preparation.
- Helps eliminate template DNA after in vitro transcription.
Specifications
| Property | Details |
|---|---|
| Enzyme Type | DNase I (RNase-Free) |
| Concentration | 1 U/μL |
| Activity Dependency | Requires Ca²⁺, activated by Mg²⁺ or Mn²⁺ |
| Cleavage Pattern | Random (Mg²⁺) or site-specific (Mn²⁺) |
| Product Type | Endonuclease |
| Quantity | 1,000 Units |
| Storage Condition | Store at -20°C |
| Compatible Buffer | DNase I Reaction Buffer |
| Formulation | Recombinant, non-animal sourced |
| Handling Precaution | Do not vortex; mix gently by inverting |
Applications
1. RNA Preparation & DNA Removal
- Prepares DNA-free RNA for downstream applications.
- Removes genomic DNA contamination before RT-PCR and RT-qPCR.
- Eliminates template DNA after in vitro transcription.
2. Molecular Biology & Enzyme Assays
- Nick-translation DNA labeling, in conjunction with DNA Polymerase I.
- Footprinting assays to study DNA-protein interactions.
- Used in random DNA fragmentation for DNA library generation.
3. DNA Modification & Research
- Cleaves DNA at random positions in Mg²⁺-containing buffer.
- Produces blunt-ended or overhang DNA fragments when Mn²⁺ is present.
- Used in chromatin accessibility studies and functional genomics.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 1,000 Units | EN0521 |
Storage & Handling
- Store at -20°C to maintain enzyme activity.
- Do not vortex; mix gently by inverting the tube.
- Always use sterile pipetting techniques to prevent contamination.
Thermo Scientific™ DNase I, RNase-Free (1 U/μL) is a high-purity, recombinant endonuclease ideal for DNA degradation, RNA purification, and molecular biology applications. It is RNase-free, ensuring RNA integrity, and provides flexible cleavage patterns based on cationic conditions. Its versatility and specificity make it a valuable tool for DNA and RNA research.
DNase I, RNase-free - Product Information
User Guide: DNase I, RNase-free, 1U/uL
Thermo Scientific™ DNase I, RNase-Free is a high-purity endonuclease that digests single- and double-stranded DNA, breaking down phosphodiester bonds to produce mono- and oligodeoxyribonucleotides with 5′-phosphate and 3′-OH groups. The enzyme is recombinant and purified from a non-animal host, ensuring minimal intrinsic RNase contamination, making it ideal for RNA preparation and molecular biology applications.
The enzyme’s activity is strictly dependent on Ca²⁺ ions and is activated by Mg²⁺ or Mn²⁺ ions, allowing for different cleavage patterns depending on the cation present:
- Mg²⁺: Cleaves each DNA strand independently, generating randomly fragmented DNA.
- Mn²⁺: Cleaves both DNA strands at the same site, producing blunt-ended or slightly overhanging DNA fragments.
Key Features & Benefits
1. High-Purity Recombinant Enzyme
- RNase-free, ensuring DNA digestion without affecting RNA integrity.
- Produced in a non-animal host, reducing contamination risks.
- High specificity and efficiency for DNA degradation.
2. Versatile DNA Digestion Capabilities
- Cleaves single- and double-stranded DNA into short fragments.
- Generates blunt or overhanging DNA ends, depending on divalent cation conditions.
- Works in a variety of reaction buffers, enhancing compatibility with different workflows.
3. Optimized for RNA Preparation & Molecular Biology
- Removes DNA contamination from RNA samples before RT-PCR or RT-qPCR.
- Suitable for DNA footprinting, nick-translation, and DNA library preparation.
- Helps eliminate template DNA after in vitro transcription.
Specifications
| Property | Details |
|---|---|
| Enzyme Type | DNase I (RNase-Free) |
| Concentration | 1 U/μL |
| Activity Dependency | Requires Ca²⁺, activated by Mg²⁺ or Mn²⁺ |
| Cleavage Pattern | Random (Mg²⁺) or site-specific (Mn²⁺) |
| Product Type | Endonuclease |
| Quantity | 1,000 Units |
| Storage Condition | Store at -20°C |
| Compatible Buffer | DNase I Reaction Buffer |
| Formulation | Recombinant, non-animal sourced |
| Handling Precaution | Do not vortex; mix gently by inverting |
Applications
1. RNA Preparation & DNA Removal
- Prepares DNA-free RNA for downstream applications.
- Removes genomic DNA contamination before RT-PCR and RT-qPCR.
- Eliminates template DNA after in vitro transcription.
2. Molecular Biology & Enzyme Assays
- Nick-translation DNA labeling, in conjunction with DNA Polymerase I.
- Footprinting assays to study DNA-protein interactions.
- Used in random DNA fragmentation for DNA library generation.
3. DNA Modification & Research
- Cleaves DNA at random positions in Mg²⁺-containing buffer.
- Produces blunt-ended or overhang DNA fragments when Mn²⁺ is present.
- Used in chromatin accessibility studies and functional genomics.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 1,000 Units | EN0521 |
Storage & Handling
- Store at -20°C to maintain enzyme activity.
- Do not vortex; mix gently by inverting the tube.
- Always use sterile pipetting techniques to prevent contamination.
Thermo Scientific™ DNase I, RNase-Free (1 U/μL) is a high-purity, recombinant endonuclease ideal for DNA degradation, RNA purification, and molecular biology applications. It is RNase-free, ensuring RNA integrity, and provides flexible cleavage patterns based on cationic conditions. Its versatility and specificity make it a valuable tool for DNA and RNA research.