Thermo Scientific™ Oxoid™ Baird-Parker RPF Agar Base

The Thermo Scientific™ Oxoid™ Baird-Parker RPF Agar Base is a highly selective medium designed for the isolation and enumeration of coagulase-positive staphylococci, including Staphylococcus aureus, from food samples. This medium is specifically formulated for use with the RPF Supplement (Part No. SR0122A), providing enhanced diagnostic accuracy while eliminating the need for egg yolk tellurite emulsion.

Key Features

1. Selective for Staphylococci:
   • Formulated to selectively isolate coagulase-positive staphylococci in complex food matrices.
2. RPF Supplement Compatibility:
   • Requires RPF Supplement (Part No. SR0122A) for diagnostic efficacy.
   • Eliminates the need for egg yolk tellurite emulsion, simplifying preparation.
3. Regulatory Compliance:
   • Meets international standards such as ISO and NMKL, ensuring reliability in food safety testing.
4. High Yield:
   • Each 500g package yields 7.1L of prepared medium, ideal for high-volume testing.

Applications

1. Food Microbiology

• Detection and enumeration of coagulase-positive staphylococci in food products, including dairy, meat, and processed foods.
• Routine food safety testing for regulatory compliance.

2. Public Health and Regulatory Testing

• Ensures food safety in alignment with ISO and NMKL standards for foodborne pathogen detection.

3. Industrial Microbiology

• Used in food production facilities to monitor contamination and ensure microbial quality assurance.

Specifications

Preparation Instructions

1. Suspend: Suspend 70.4g of Baird-Parker RPF Agar Base in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation to dissolve the powder completely.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Supplement: Cool to 45–50°C and aseptically add the reconstituted RPF Supplement (Part No. SR0122A).
5. Dispense: Pour the prepared medium into sterile Petri dishes and allow to solidify.

Advantages

1. Simplified Preparation: Eliminates the need for egg yolk tellurite emulsion, reducing preparation complexity.
2. Selective and Specific: Enhances the isolation of coagulase-positive staphylococci while suppressing non-target organisms.
3. High Sensitivity: Ensures recovery of Staphylococcus aureus in food samples, even at low levels.
4. Regulatory Compliance: Aligns with ISO and NMKL standards for reliable food safety testing.
5. Cost-Effective: High yield of 7.1L per 500g package ensures economical testing.

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Baird-Parker Agar Base, RPF
• Quantity: 500g
• Yield: 7.1L of prepared medium

Regulatory and Reference Standards

• Certifications: ISO, NMKL
• Complies with internationally recognized standards for foodborne pathogen detection.

The Thermo Scientific™ Oxoid™ Baird-Parker RPF Agar Base offers a reliable and selective solution for isolating and enumerating coagulase-positive staphylococci from food samples. With its compatibility with RPF Supplement and compliance with ISO and NMKL standards, this medium is an essential tool for food safety testing, public health monitoring, and industrial microbiology.