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Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) is a highly selective medium designed for the isolation of Campylobacter species, including C. jejuni, C. coli, and C. laridis. Developed as a blood-free alternative, this medium incorporates charcoal, ferrous sulphate, and sodium pyruvate to replace blood, enhancing its usability and reliability. When supplemented with CCDA Selective Supplement SR0155, the medium becomes ideal for isolating Campylobacter from clinical, food, and environmental samples.
Key Features
- Selective Formulation:
- Contains cefoperazone and amphotericin B, ensuring selectivity by suppressing non-target organisms.
- Blood-Free Alternative:
- Replaces blood with charcoal, ferrous sulphate, and sodium pyruvate, simplifying preparation and storage.
- Recommended by Authorities:
- Endorsed by the U.K. Ministry of Agriculture, Fisheries and Food (MAFF) for Campylobacter isolation.
- Supports Species Identification:
- Colonial morphology aids in differentiating Campylobacter species:
- C. jejuni: Grey, moist, flat, spreading colonies (sometimes with a metallic sheen).
- C. coli: Creamy-grey, moist, slightly raised colonies.
- Colonial morphology aids in differentiating Campylobacter species:
- Versatile Application:
- Suitable for testing fecal samples, food, and environmental matrices.
Applications
1. Clinical Microbiology
- Isolation of Campylobacter spp. from fecal samples in diagnostic workflows.
2. Food Microbiology
- Detection of Campylobacter in food products, including:
- Meat and poultry
- Seafood
- Dairy products
- Ready-to-eat foods
3. Environmental Microbiology
- Monitoring Campylobacter contamination in water, soil, and food production facilities.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Campylobacter Blood-Free Selective Agar Base |
| Selective Agents | Cefoperazone, Amphotericin B |
| Quantity | 500g |
| Yield | 11.0L of prepared medium |
| Applications | Clinical, food, and environmental testing |
Preparation Instructions
- Suspend: Suspend 45g of Campylobacter Blood-Free Selective Agar Base in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Supplement: After cooling to 45–50°C, aseptically add CCDA Selective Supplement SR0155.
- Dispense: Pour into sterile Petri dishes and allow the medium to solidify.
Advantages
- Blood-Free Design: Eliminates the need for blood, simplifying preparation and enhancing stability.
- Selective and Effective: Optimized to suppress non-target organisms while promoting Campylobacter growth.
- High Reliability: Validated by recognized authorities for use in food safety and clinical diagnostics.
- Clear Identification: Colonial morphology supports species-level differentiation.
- Versatile Use: Suitable for clinical, food, and environmental microbiology workflows.
Diagnostic Features
| Component | Function |
|---|---|
| Charcoal | Neutralizes toxic compounds. |
| Ferrous Sulphate | Enhances microbial growth. |
| Sodium Pyruvate | Promotes recovery of injured cells. |
| Cefoperazone | Suppresses non-target bacteria. |
| Amphotericin B | Inhibits fungal contamination. |
Colonial Morphology
| Species | Colony Appearance |
|---|---|
| Campylobacter jejuni | Grey, moist, flat, spreading colonies (green hue or metallic sheen possible). |
| Campylobacter coli | Creamy-grey, moist, slightly raised colonies. |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Campylobacter Blood-Free Selective Agar Base (Dehydrated)
- Quantity: 500g
- Yield: 11.0L of prepared medium
The Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base is a reliable and effective medium for isolating and identifying Campylobacter spp. in diverse sample types. Its blood-free formulation, selective agents, and broad application range make it a valuable tool for microbiology laboratories focused on food safety, clinical diagnostics, and environmental monitoring.