Thermo Scientific™ Oxoid™ Yersinia Selective Agar Base (Dehydrated)

The Thermo Scientific™ Oxoid™ Yersinia Selective Agar Base (Dehydrated) is a specialized medium designed for the isolation and enumeration of Yersinia enterocolitica from clinical specimens and food samples. When supplemented with Yersinia Selective Supplement (SR0109), this medium ensures optimal growth and recovery of Yersinia enterocolitica, a pathogen associated with gastrointestinal and systemic infections.

Key Features

1. Highly Selective for Yersinia enterocolitica:
   • Specifically formulated to promote the growth of Yersinia enterocolitica while inhibiting competing flora.
2. Distinctive Colony Morphology:
   • Yersinia enterocolitica colonies appear as red bull’s-eye centers surrounded by transparent borders.
   • Differentiation from other organisms such as Serratia liquefaciens and Citrobacter freundii is achieved through biochemical tests.
3. Optimal Growth Conditions:
   • Designed for incubation at 32°C for 18-24 hours for reliable recovery.
4. Versatile Applications:
   • Suitable for clinical diagnostic testing of fecal samples and for food microbiology.

Specifications

Applications

1. Clinical Microbiology:
   • Isolate and identify Yersinia enterocolitica from fecal samples to diagnose gastrointestinal or systemic infections.
2. Food Microbiology:
   • Detect Yersinia enterocolitica in food products, especially those associated with outbreaks of foodborne illness.
3. Research:
   • Study the colony morphology, biochemical properties, and behavior of Yersinia enterocolitica in controlled environments.

Preparation Instructions

1. Suspend 58g of Yersinia Selective Agar Base powder in 1 liter of distilled water.
2. Heat gently to dissolve completely.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 50°C, and aseptically add the reconstituted Yersinia Selective Supplement (SR0109) as directed.
5. Mix thoroughly, pour into sterile Petri dishes, and allow to solidify.

Colony Characteristics

• Yersinia enterocolitica: Red bull’s-eye center surrounded by a transparent border.
• Other organisms: Larger colonies (>2mm) with diffuse pinkish centers and opaque outer zones.

Advantages

• Selective and Specific: Promotes Yersinia enterocolitica growth while inhibiting non-target organisms.
• Clear Differentiation: Distinct colony morphology aids in presumptive identification.
• Versatile Use: Effective for both clinical and food microbiology applications.
• Time-Efficient: Delivers reliable results after 18-24 hours of incubation.

Packaging

• Quantity: 500g of dehydrated powder.
• Yield: Prepares 8.6L of medium.

Why Choose Yersinia Selective Agar Base?

• Optimized for the selective isolation of Yersinia enterocolitica from diverse sample types.
• Trusted by laboratories for clinical diagnostics and food safety testing.
• Backed by Thermo Scientific’s expertise in microbiological media development.