Thermo Scientific™ Oxoid™ Pseudomonas Agar Base (Dehydrated).

The Thermo Scientific™ Oxoid™ Pseudomonas Agar Base (Dehydrated) is a selective medium designed for the isolation and differentiation of Pseudomonas species from a variety of clinical, food, water, and environmental samples. Based on the modification of King's A Medium, it incorporates magnesium chloride and potassium sulfate to enhance pigment production and improve recovery of Pseudomonas aeruginosa. When supplemented with the appropriate additives (SR0102 or SR0103), the medium can be tailored for specific applications.

Key Features

1. Selective and Specific:
   • Supports the growth of Pseudomonas aeruginosa while strongly suppressing the growth of competing organisms like Klebsiella, Proteus, and Providencia spp.
2. Enhanced Identification:
   • Encourages pigment production for easy differentiation:
     • Pseudomonas aeruginosa: Fluorescent green colonies.
3. Versatile Applications:
   • Suitable for clinical, food, water, and environmental testing when supplemented appropriately.
4. Supplementation Options:
   • SR0102: Makes the medium selective for Pseudomonas aeruginosa.
   • SR0103: Makes the medium selective for Pseudomonas spp. generally.

Specifications

Applications

1. Clinical Microbiology:
   • Isolate and differentiate Pseudomonas aeruginosa and other Pseudomonas species from clinical samples.
2. Food and Beverage Testing:
   • Detect and enumerate Pseudomonas species in processed and raw food products.
3. Water and Environmental Testing:
   • Identify Pseudomonas species in water and environmental samples for quality control and research.

Preparation Instructions

1. Suspend 45.5g of Pseudomonas Agar Base powder in 1 liter of distilled water.
2. Heat to dissolve completely.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool to 50°C, and aseptically add the appropriate selective supplement (SR0102 or SR0103) as directed.
5. Mix thoroughly and pour into sterile Petri dishes.

Advantages

• Highly Selective: Strongly suppresses non-target organisms for improved specificity.
• Reliable Identification: Enhanced pigment production facilitates visual differentiation.
• Flexible Use: Adaptable for various sample types through supplementation.
• Proven Performance: Based on King's A Medium, a well-established formula for Pseudomonas recovery.

Packaging

• Quantity: 500g of dehydrated powder.
• Yield: Prepares 10.3L of medium.

Why Choose Pseudomonas Agar Base?

• Tailored for the selective isolation and differentiation of Pseudomonas species.
• Widely used in clinical, food, water, and environmental microbiology.
• Backed by Thermo Scientific’s commitment to quality and innovation.